Aberration-accounting calibration for 3D single-molecule localization microscopy
Aberration-accounting calibration for 3D single-molecule localization microscopy
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DOI：
10.1364/ol.43.000174
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发表时间：
2018-01-15
期刊：
影响因子：
3.6
通讯作者：
Leveque-Fort, Sandrine
中科院分区：
文献类型：
--
作者：
Cabriel, Clement;Bourg, Nicolas;Leveque-Fort, Sandrine
我们提出了一种简单的基于样本的技术来校准 3D 单分子定位显微镜中的轴向检测。使用涂有荧光分子的微球，可以在成像深度范围内获得点扩散函数整形或基于强度的测量的校准曲线。该实验方法考虑了球面像差的影响，无需计算校正。我们展示了其在盖玻片上方 1.2 μm 范围内进行像散成像的效率。 (c) 2018 年美国光学学会。
We propose a straightforward sample-based technique to calibrate the axial detection in 3D single-molecule localization microscopy. Using microspheres coated with fluorescent molecules, the calibration curves of point spread function-shaping or intensity-based measurements can be obtained over the imaging depth range. This experimental method takes into account the effect of the spherical aberration without requiring computational correction. We demonstrate its efficiency for astigmatic imaging in a 1.2 mu m range above the coverslip. (c) 2018 Optical Society of America.