Comparison of the Proliferation, Viability, and Differentiation Capacity of Adipose-Derived Stem Cells from Different Anatomic Sites in Rabbits
Comparison of the Proliferation, Viability, and Differentiation Capacity of Adipose-Derived Stem Cells from Different Anatomic Sites in Rabbits
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DOI：
10.1159/000330796
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发表时间：
2012-01-01
影响因子：
2.7
通讯作者：
Ye, Z. Q.
中科院分区：
文献类型：
--
作者：
Chen, L.;Peng, E. J.;Ye, Z. Q.
组织工程在临床上对于缺失和受损的组织有希望。脂肪源性干细胞（ASC）是间充质干细胞的一种，是组织工程种子细胞的可靠来源，具有侵入性小、产量丰富、免疫力低、发病率低、易于分离、扩增快速等优点。然而，由于脂肪组织来源的细胞的特性根据其来源的脂肪库而有所不同，因此我们比较了新西兰白兔三个解剖部位的 ASC：腹股沟皮下 (SI)、背颈皮下 (SD) 和腹膜后肾周 (RP) 区域。我们研究了细胞行为，包括增殖、活力和分化。与 RP 区域相比，皮下区域（SI 和 SD）的 ASC 在所有评估中都具有更高的性能。此外，SI和SD ASC存在显着差异，SI ASC比SD ASC具有更好的性能。我们得出的结论是，脂肪的不同解剖分布导致了 ASC 的不同行为。 SI 区域为 ASC 组织工程提供了最适用的细胞源库。版权所有 (C) 2011 S. Karger AG，巴塞尔
Tissue engineering is clinically promising for missing and damaged tissues. Adipose-derived stem cells (ASCs), a type of mesenchymal stem cells, represent a reliable source of seed cells for tissue engineering with multiple merits such as minimal invasion, abundant yield, little immunity, low morbidity, easy isolation, and rapid expansion. However, because the properties of adipose tissue-derived cells differ depending on the fat depot from which they are derived, we compared the ASCs from three anatomic sites of New Zealand white rabbits: subcutaneous inguinal (SI), subcutaneous dorsocervical (SD), and retroperitoneal perinephric (RP) regions. We investigated cellular behaviors including proliferation, viability, and differentiation. The ASCs of the subcutaneous regions (SI and SD) had higher performances in all assessments compared to those of the RP region. Moreover, the SI and SD ASCs had significant differences, with SI ASCs having better properties than SD ASCs. We conclude that the different anatomic distributions of fat contribute to the different behaviors of ASCs. The SI region offers the most applicable cell source reservoir for ASC tissue engineering. Copyright (C) 2011 S. Karger AG, Basel