Possible function for virus encoded K+ channel Kcv in the replication of chlorella virus PBCV-1
Possible function for virus encoded K+ channel Kcv in the replication of chlorella virus PBCV-1
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DOI：
10.1016/s0014-5793(03)00776-2
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发表时间：
2003-09-18
期刊：
影响因子：
3.5
通讯作者：
Thiel, G
中科院分区：
文献类型：
--
作者：
Mehmel, M;Rothermel, M;Thiel, G
K+通道Kcv由雷氏杆菌病毒PBCV-1编码。有证据表明，该通道在病毒复制中起着重要作用，因为爪蟾卵母细胞中的PBCV-1噬斑形成和Kcv通道活性对抑制剂具有相似的敏感性。在这里，我们报告间接证据表明，KCV通道是重要的病毒感染过程中。在宿主细胞小球藻NC 64 A中的膜电压的记录揭示了在感染的最初几分钟内的膜去极化。这种去极化显示出与Kcv电导相同的阳离子敏感性;去极化还需要病毒体的完整膜。总之，这些数据与病毒在病毒体中携带功能性K+通道并在感染期间将其插入宿主细胞质膜的想法一致。(C)2003年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
The K+ channel Kcv is encoded by the chlorella virus PBCV-1. There is evidence that this channel plays an essential role in the replication of the virus, because both PBCV-1 plaque formation and Kcv channel activity in Xenopus oocytes have similar sensitivities to inhibitors. Here we report circumstantial evidence that the Kcv channel is important during virus infection. Recordings of membrane voltage in the host cells Chlorella NC64A reveal a membrane depolarization within the first few minutes of infection. This depolarization displays the same sensitivity to cations as Kcv conductance; depolarization also requires the intact membrane of the virion. Together these data are consistent with the idea that the virus carries functional K+ channels in the virion and inserts them into the host cell plasma membrane during infection. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.