Fast and compact DNA logic circuits based on single-stranded gates using strand-displacing polymerase
Fast and compact DNA logic circuits based on single-stranded gates using strand-displacing polymerase
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DOI：
10.1038/s41565-019-0544-5
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发表时间：
2019-11-01
影响因子：
38.3
通讯作者：
Reif, John
中科院分区：
文献类型：
--
作者：
Song, Tianqi;Eshra, Abeer;Reif, John
DNA是构建分子计算系统的可靠生物分子。特别是，DNA逻辑电路(基于扩散)在计算的可扩展性和正确性方面表现出了良好的性能。然而，以前的DNA逻辑电路的体系结构有两个局限性。首先，计算速度很慢，通常需要数小时才能计算出一个简单的函数。其次，关于DNA链的数量，电路具有很高的复杂性。在这里，我们介绍了一种基于单链逻辑门的DNA逻辑电路的体系结构，该逻辑门使用链置换DNA聚合酶。逻辑门只由单DNA链组成，这极大地减少了泄漏反应和信号恢复步骤，从而使电路在计算速度和所需DNA链数量方面都得到了改进。大规模逻辑电路可以通过简单的级联策略从门电路中构造出来。特别是，我们演示了一种快速而紧凑的逻辑电路，它可以计算四位输入数的平方根函数。
DNA is a reliable biomolecule with which to build molecular computation systems. In particular, DNA logic circuits (diffusion-based) have shown good performance regarding scalability and correctness of computation. However, previous architectures of DNA logic circuits have two limitations. First, the speed of computation is slow, often requiring hours to compute a simple function. Second, the circuits are of high complexity regarding the number of DNA strands. Here, we introduce an architecture of DNA logic circuits based on single-stranded logic gates using strand-displacing DNA polymerase. The logic gates consist of only single DNA strands, which largely reduces leakage reactions and signal restoration steps such that the circuits are improved in regard to both speed of computation and the number of DNA strands needed. Large-scale logic circuits can be constructed from the gates by simple cascading strategies. In particular, we have demonstrated a fast and compact logic circuit that computes the square-root function of four-bit input numbers.