Protective effect of HwangRyunHaeDok-Tang water extract against chronic obstructive pulmonary disease induced by cigarette smoke and lipopolysaccharide in a mouse model
Protective effect of HwangRyunHaeDok-Tang water extract against chronic obstructive pulmonary disease induced by cigarette smoke and lipopolysaccharide in a mouse model
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DOI：
10.1016/j.jep.2017.02.027
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发表时间：
2017-03-22
影响因子：
5.4
通讯作者：
Shin, In-Sik
中科院分区：
文献类型：
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作者：
Shin, Na-Rae;Ko, Je-Won;Shin, In-Sik
民族药理学相关性：Hwangryunhaedok-tang是一种东方草药配方，用于治疗中国，日本和韩国的炎症和胃病。我们探讨了对香烟烟雾（CS）和脂多糖（LPS.Materials）引起的小鼠气道病理生理变化的保护作用，黄龙海德汤水提取物（HRWE）和方法：我们进行了定量分析的五个标志成分，即栀子苷，黄芩苷，黄连碱，plamatine，小檗碱，使用高效液相色谱法。动物接受CS暴露（每天1小时）7天。在第4天鼻内施用LPS。结果：HRWE能显著抑制CS和LPS诱导的炎症细胞计数的增加。此外，HRWE治疗后观察到支气管肺泡灌洗液（BALF）中IL-6、TNF-α和IL-1 β的减少。结论：HRWE能减轻CS和LPS所致的呼吸道炎症反应，降低肺组织iNOS、NF-κ B B和MMP-9的表达。因此，HRWE具有治疗气道炎性疾病的潜力。
Ethnopharmacological relevance: Hwangryunhaedok-tang is an oriental herbal formula treated to cure inflammation and gastric disorders in China, Japan, and Korea. We explored the protective effects of Hwangryunhaedok-tang water extract (HRWE) against airway pathophysiological changes caused by cigarette smoke (CS) and lipopolysaccharide (LPS) in a mouse.Materials and methods: We performed quantitative analyses of five marker components, namely geniposide, baicalin, coptisine, plamatine, and berberine, using high-performance liquid chromatography. Animals were received CS exposure (1 h per day) for 7 days. LPS was administered intranasally on day 4. Mice were received HRWE at dose of 100 or 200 mg/kg for 1 h before CS exposure.Results: Treatment with HRWE significantly suppressed the increased inflammatory cell count induced by CS and LPS exposure. In addition, reduction in IL-6, TNF-alpha and IL-1 beta in broncho-alveolar lavage fluid (BALF) was observed after HRWE treatment. HRWE not only decreased inflammatory cell infiltration in lung, but also decreased the expression of iNOS, NF-kappa B and matrix metallopeptidase (MMP)-9 in lung tissues.Conclusion: This study showed that HRWE can attenuate respiratory inflammation caused by CS and LPS exposure. Therefore, HRWE has potential for treating airway inflammatory disease.