Microbial quality of irrigation water used in leafy green production in Southern Brazil and its relationship with produce safety
Microbial quality of irrigation water used in leafy green production in Southern Brazil and its relationship with produce safety
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DOI：
10.1016/j.fm.2017.02.003
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发表时间：
2017-08-01
影响因子：
5.3
通讯作者：
Tondo, Eduardo Cesar
中科院分区：
文献类型：
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作者：
Decol, Luana Tombini;Casarin, Leticia Sopena;Tondo, Eduardo Cesar
灌溉水被认为是许多生产地区水果和蔬菜的重要微生物风险因素，但仍然缺乏关于不同灌溉水源和气候条件的微生物质量如何影响巴西生产的蔬菜安全的信息。本研究评价了E.大肠杆菌的感染率和大肠杆菌的感染率。盘管O 157：H7存在于巴西南部用于灌溉的两种不同水源（池塘和与农田和城市地区接壤的溪流）和商业化生产的甘蔗上。我们还评估了农业因素和气象条件对水和农产品样品潜在污染的影响。在一年内（2014年7月至2015年8月）对四个农场进行了纵向研究。结果表明，E.大肠杆菌在灌溉水中的检出率为84.8%，在油菜上的检出率为38.3%，表明灌溉水是油菜的重要污染源。E.两种不同的地表水源之间的大肠杆菌。气候条件，特别是降雨和环境温度，影响了E。线圈E.在一年中最温暖的时候，在灌溉水和灌木上发现了线圈。E.用定性聚合酶链反应（qPCR）检测了13份水样中的O 157：H7大肠杆菌，但仅4份经培养基分离证实。(C)2017爱思唯尔有限公司版权所有
Irrigation water has been recognized as an important microbial risk factor for fruits and vegetables in many production areas, but there is still a lack of information about how the microbiological quality of different irrigation water sources and climatic conditions influence the safety of vegetables produced in Brazil. This study evaluated the distribution of generic E. coli and the prevalence of E. coil O157:H7 in two different water sources (ponds and streams bordering farmlands and urban areas) used for irrigation and on commercially produced lettuces in Southern Brazil. We also evaluated the effect of agricultural factors and meteorological conditions in the potential contamination of water and produce samples. A longitudinal study was conducted on four farms during a year (July 2014 to August 2015). The results showed generic E. coli prevalence of 84.8% and 38.3% in irrigation water samples and on lettuces, respectively, indicating irrigation water as an important source of contamination of lettuces. No significant differences were detected in the counts of E. coli between the two different surface water sources. The climatic conditions, particularly rainfall and environmental temperature, have influenced the high concentration of E. coil. The highest loads of E. coil in irrigation water and on lettuces were found during the warmest time of the year. E. coli O157:H7 was detected by qualitative polymerase chain reaction (qPCR) in 13 water samples but only 4 were confirmed by isolation in culture media. (C) 2017 Elsevier Ltd. All rights reserved.