CENTRACTIN IS AN ACTIN HOMOLOG ASSOCIATED WITH THE CENTROSOME
CENTRACTIN IS AN ACTIN HOMOLOG ASSOCIATED WITH THE CENTROSOME
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DOI：
10.1038/359246a0
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发表时间：
1992-09-17
期刊：
影响因子：
64.8
通讯作者：
MEYER, DI
中科院分区：
文献类型：
--
作者：
CLARK, SW;MEYER, DI
肌动蛋白是真核生物中最普遍、最丰富且保守的蛋白质之一，参与许多重要的细胞过程，包括维持细胞形状、运动和细胞分裂1,2。来自最不同来源的肌动蛋白仍然具有超过 70% 的氨基酸同一性（参考文献 3）。这可以很好地解释为什么肌动蛋白的低丰度同系物难以分离。编码芽殖酵母和裂殖酵母中肌动蛋白远亲的基因现已被克隆3,4。我们在这里报告了一种脊椎动物肌动蛋白样蛋白的发现，我们将其命名为 centractin。分离出全长互补 DNA 克隆，其序列显示与肌动蛋白的氨基酸同一性超过 50%，当考虑保守氨基酸变化时，该同一性增加到 70% 以上。 Northern 分析和蛋白质印迹表明普遍存在的组织和物种分布。形态学和生化标准表明 Centractin 与中心体相关。
ACTIN is one of the most ubiquitous, abundant and well-conserved proteins of eukaryotes, participating in many crucial cellular processes including the maintenance of cell shape, motility and cell division1,2. Actins from the most divergent sources still share amino-acid identities in excess of 70% (ref. 3). This may well explain why low-abundance homologues of actin have been difficult to isolate. Genes encoding distant relatives of actin in budding and fission yeast have now been cloned3,4. We report here the discovery of a vertebrate actin-like protein, which we name centractin. A full-length complementary DNA clone was isolated whose sequence reveals amino-acid identities with actin of over 50%, increasing to more than 70% when conservative amino-acid changes are considered. Northern analysis and western blotting indicate a ubiquitous tissue and species distribution. Morphological and biochemical criteria show that centractin is associated with centrosomes.