A mitotic septin scaffold required for mammalian chromosome congression and segregation
A mitotic septin scaffold required for mammalian chromosome congression and segregation
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DOI：
10.1126/science.1106823
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发表时间：
2005-03-18
期刊：
影响因子：
56.9
通讯作者：
Nelson, WJ
中科院分区：
文献类型：
--
作者：
Spiliotis, ET;Kinoshita, M;Nelson, WJ
胞质分裂与染色体聚集和分离的协调是细胞正常分裂的关键，但其机制尚不清楚。在这里，septins，一个保守的家庭聚合鸟苷三磷酸结合蛋白，定位于中期板在有丝分裂。Septin耗竭导致染色体从中期板丢失，缺乏染色体分离和纺锤体伸长，以及延迟有丝分裂退出时的不完全胞质分裂。这些缺陷与有丝分裂运动和检查点调节器着丝粒相关蛋白E（CENP-E）从聚集染色体的动粒的损失。因此，哺乳动物septins可以形成CENP-E和其他效应子的有丝分裂支架，以协调胞质分裂与染色体聚集和分离。
Coordination of cytokinesis with chromosome congression and segregation is critical for proper cell division, but the mechanism is unknown. Here, septins, a conserved family of polymerizing guanosine triphosphate-binding proteins, localized to the metaphase plate during mitosis. Septin depletion resulted in chromosome loss from the metaphase plate, lack of chromosome segregation and spindle elongation, and incomplete cytokinesis upon delayed mitotic exit. These defects correlated with loss of the mitotic motor and the checkpoint regulator centromere-associated protein E (CENP-E) from the kinetochores of congressing chromosomes. Mammalian septins may thus form a mitotic scaffold for CENP-E and other effectors to coordinate cytokinesis with chromosome congression and segregation.