Assessment of the Radiation Effects of Cardiac CT Angiography Using Protein and Genetic Biomarkers.
Assessment of the Radiation Effects of Cardiac CT Angiography Using Protein and Genetic Biomarkers.
复制标题
DOI：
10.1016/j.jcmg.2015.04.016
复制
发表时间：
2015-08
期刊：
影响因子：
--
通讯作者：
Wu JC
中科院分区：
文献类型：
--
作者：
Nguyen PK;Lee WH;Li YF;Hong WX;Hu S;Chan C;Liang G;Nguyen I;Ong SG;Churko J;Wang J;Altman RB;Fleischmann D;Wu JC
评估心脏计算机断层扫描血管造影的辐射暴露是否与 DNA 损伤有关，以及损伤是否导致程序性细胞死亡以及参与细胞凋亡和 DNA 修复的基因激活。暴露于医学成像辐射已成为一个公共健康问题，但它是否会导致严重的细胞损伤仍不清楚。我们对 2012 年 1 月至 2013 年 12 月期间在美国两个医疗中心接受心脏计算机断层扫描 (CTA) 的 67 名患者进行了一项前瞻性队列研究。使用体模剂量测定法估计中位血液辐射暴露。通过流式细胞术、全基因组测序和单细胞聚合酶链式反应测量 DNA 损伤和细胞凋亡的生物标志物。中位 DLP 为 1535.3 mGy·cm (969.7 – 2674.0 mGy·cm)。血液的中位辐射剂量为 29.8 毫西弗 (18.8 – 48.8 mSv)。辐射后 DNA 损伤中位数增加了 3.39% (1.29 – 8.04%, P<0.0001)。辐射后细胞凋亡中位数增加 3.1 倍（1.4 – 5.1 倍，P<0.0001）。全基因组测序揭示了参与细胞凋亡、细胞周期和 DNA 修复调节的 39 种转录因子的表达变化。参与介导细胞凋亡和DNA修复的基因在辐射后发生显着变化，包括DDB2[1.9倍（1.5-3.0倍），P<0.001]、XRCC4[3.0倍（1.1-5.4倍），P=0.005]和BAX[1.6倍（0.9-2.6倍），P<0.001]。暴露于辐射与 DNA 损伤相关 [OR: 1.8 (1.2 – 2.6), P=0.003]。 DNA 损伤与细胞凋亡 [OR: 1.9 (1.2 – 5.1), P<0.0001] 和基因激活 [OR: 2.8 (1.2 – 6.2), P=0.002] 相关。暴露于心脏 CTA 辐射的患者有 DNA 损伤的证据，这与程序性细胞死亡以及参与细胞凋亡和 DNA 修复的基因激活有关。
To evaluate whether radiation exposure from cardiac computed tomographic angiography is associated with DNA damage and whether damage leads to programmed cell death and activation of genes involved in apoptosis and DNA repair. Exposure to radiation from medical imaging has become a public health concern, but whether it causes significant cell damage remains unclear. We conducted a prospective cohort study in 67 patients undergoing cardiac computed tomographic angiography (CTA) between January 2012 and December 2013 in two US medical centers. Median blood radiation exposure was estimated using phantom dosimetry. Biomarkers of DNA damage and apoptosis were measured by flow cytometry, whole genome sequencing, and single cell polymerase chain reaction. The median DLP was 1535.3 mGy·cm (969.7 – 2674.0 mGy·cm). The median radiation dose to the blood was 29.8 milliSieverts (18.8 – 48.8 mSv). Median DNA damage increased 3.39% (1.29 – 8.04%, P<0.0001) post-radiation. Median apoptosis increased 3.1-fold (1.4 – 5.1-fold, P<0.0001) post-radiation. Whole genome sequencing revealed changes in the expression of 39 transcription factors involved in the regulation of apoptosis, cell cycle, and DNA repair. Genes involved in mediating apoptosis and DNA repair were significantly changed post-radiation, including DDB2 [1.9-fold (1.5 – 3.0-fold), P<0.001], XRCC4 [3.0-fold (1.1 – 5.4-fold), P=0.005], and BAX [1.6-fold (0.9 – 2.6-fold), P<0.001]. Exposure to radiation was associated with DNA damage [OR: 1.8 (1.2 – 2.6), P=0.003]. DNA damage was associated with apoptosis [OR: 1.9 (1.2 – 5.1), P<0.0001] and gene activation [OR: 2.8 (1.2 – 6.2), P=0.002]. Patients exposed to radiation from cardiac CTA had evidence of DNA damage, which was associated with programmed cell death and activation of genes involved in apoptosis and DNA repair.
登录
查看更多内容
DOI：
10.1073/pnas.0501895102
发表时间：
2005-06-21
影响因子：
11.1
作者：
Löbrich, M;Rief, N;Uder, M
通讯作者：
Uder, M
DOI：
10.1056/nejmoa0901249
发表时间：
2009-08-27
期刊：
The New England journal of medicine
影响因子：
--
作者：
Fazel R;Krumholz HM;Wang Y;Ross JS;Chen J;Ting HH;Shah ND;Nasir K;Einstein AJ;Nallamothu BK
通讯作者：
Nallamothu BK
影响因子：
37.8
作者：
Beels, Laurence;Bacher, Klaus;Thierens, Hubert
通讯作者：
Thierens, Hubert
影响因子：
19.7
作者：
Little, Mark P.;Wakeford, Richard;de Gonzalez, Amy Berrington
通讯作者：
de Gonzalez, Amy Berrington
影响因子：
19.7
作者：
Einstein, Andrew J.;Elliston, Carl D.;Brenner, David J.
通讯作者：
Brenner, David J.