Zebrafish wnt8 encodes two wnt8 proteins on a bicistronic transcript and is required for mesoderm and neurectoderm patterning
Zebrafish wnt8 encodes two wnt8 proteins on a bicistronic transcript and is required for mesoderm and neurectoderm patterning
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DOI：
10.1016/s1534-5807(01)00007-7
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发表时间：
2001-07-01
影响因子：
11.8
通讯作者：
Moon, RT
中科院分区：
文献类型：
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作者：
Lekven, AC;Thorpe, CJ;Moon, RT
在脊椎动物中，wnt 8与中胚层的早期模式有关。为了直接确定胚胎对wnt 8的需求，我们在斑马鱼中产生了染色体缺陷，去除了双顺反子wnt 8基因座。我们报告说，纯合子突变体表现出明显的缺陷，在背腹侧中胚层图案和前后神经模式。尽管它们的信号传导活性存在差异，但双顺反子RNA的任一编码区都可以挽救缺陷表型。吗啉代反义寡聚体特异性干扰wnt8翻译，表型复制缺陷，干扰ntl和spt突变体中的wnt8翻译，产生缺少躯干和尾部的胚胎。这些数据表明，斑马鱼wnt8基因座需要在原肠胚形成中胚层和神经外胚层适当的模式。
In vertebrates, wnt8 has been implicated in the early patterning of the mesoderm. To determine directly the embryonic requirements for wnt8, we generated a chromosomal deficiency in zebrafish that removes the bicistronic wnt8 locus. We report that homozygous mutants exhibit pronounced defects in dorso-ventral mesoderm patterning and in the antero-posterior neural pattern. Despite differences in their signaling activities, either coding region of the bicistronic RNA can rescue the deficiency phenotype. Specific interference of wnt8 translation by morpholino antisense oligomers phenocopies the deficiency, and interference with wnt8 translation in ntl and spt mutants produces embryos lacking trunk and tail. These data demonstrate that the zebrafish wnt8 locus is required during gastrulation to pattern both the mesoderm and the neural ectoderm properly.