Determination of the steroid hormone levels in water samples by dispersive liquid-liquid microextraction with solidification of a floating organic drop followed by high-performance liquid chromatography
Determination of the steroid hormone levels in water samples by dispersive liquid-liquid microextraction with solidification of a floating organic drop followed by high-performance liquid chromatography
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DOI：
10.1016/j.aca.2010.01.003
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发表时间：
2010-03-03
影响因子：
6.2
通讯作者：
Huang, Shang-Da
中科院分区：
文献类型：
--
作者：
Chang, Chu-Chi;Huang, Shang-Da
在本研究中，利用基于漂浮有机液滴凝固的新型分散液-液微萃取方法（DLLME-SFO）测定了河流和自来水样品中的类固醇激素水平。优化了几个参数，包括萃取和分散溶剂的类型和体积、萃取时间和盐效应。 DLLME-SFO 是一种快速、廉价且易于使用的方法，用于检测样品的痕量水平。最重要的是，该方法使用毒性较小的溶剂。校准曲线的相关系数高于0.9991。线性范围为5至1000μgL(-1)。使用 DLLME-SFO 分析加标的环境水样。河水（E1 添加 4 μg L-1、E2 添加 3 μg L-1、EE2 添加 4 μg L-1、E3 添加 9 μg L-1）的相对回收率为 87% 至 116%，自来水（E1 添加 6 μg L-1、E2 添加 5 μg L-1、6 μg L-1）的相对回收率为 89% 至 102%。 L-1用于EE2，10μg L-1用于E3)。该方法的检出限对于加标河水为 0.8 至 2.7 μg L-1，对于加标自来水为 1.4 至 3.1 μg L-1。对于加标的河水，该方法的精度范围为 8% 至 14%；对于加标的自来水，该方法的精度范围为 7% 至 14%。 (C) 2010 Elsevier B.V. 保留所有权利。
In this study, the steroid hormone levels in river and tap water samples were determined by using a novel dispersive liquid-liquid microextraction method based on the solidification of a floating organic drop (DLLME-SFO). Several parameters were optimized, including the type and volume of the extraction and dispersive solvents, extraction time, and salt effect. DLLME-SFO is a fast, cheap, and easy-to-use method for detecting trace levels of samples. Most importantly, this method uses less-toxic solvent. The correlation coefficient of the calibration curve was higher than 0.9991. The linear range was from 5 to 1000 mu gL(-1). The spiked environmental water samples were analyzed using DLLME-SFO. The relative recoveries ranged from 87% to 116% for river water (which was spiked with 4 mu g L-1 for E1, 3 mu g L-1 for E2,4 mu g L-1 for EE2 and 9 mu g L-1 for E3) and 89% to 102% for tap water (which was spiked with 6 mu g L-1 for E1, 5 mu g L-1 for E2, 6 mu g L-1 for EE2 and 10 mu g L-1 for E3). The detection limits of the method ranged from 0.8 to 2.7 mu g L-1 for spiked river water and 1.4 to 3.1 mu g L-1 for spiked tap water. The methods precision ranged from 8% to 14% for spiked river water and 7% to 14% for spiked tap water. (C) 2010 Elsevier B.V. All rights reserved.