Reduced hydrogen peroxide production in neutrophils from patients with diabetes
Reduced hydrogen peroxide production in neutrophils from patients with diabetes
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DOI：
10.1016/0168-8227(96)01287-9
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发表时间：
1996-07-01
影响因子：
5.1
通讯作者：
Tsuji, M
中科院分区：
文献类型：
--
作者：
Inoue, S;Lan, Y;Tsuji, M
应用流式细胞仪检测了粒细胞集落刺激因子(G-CSF)、粒细胞-巨噬细胞集落刺激因子(GM-CSF)和佛波酯(PMA)对糖尿病(DM)患者中性粒细胞产生H_2O_2的影响。选择22名年龄匹配的男性受试者：11名为正常志愿者，其余为糖尿病患者。在DM患者的中性粒细胞中，每60分钟细胞内过氧化氢的产生没有显著差异(37.2+/-20.4A.U..)与正常志愿者(24.9+/-8.4 A.U..)比较。与糖尿病患者相比，正常志愿者的PMA刺激的中性粒细胞每60分钟产生的过氧化氢大约增加4倍。在相似的培养条件下，G-CSF使正常人中性粒细胞产生的H_2O_2是DM患者的1.6倍。在GM-CSF刺激后，志愿者中性粒细胞的增加是糖尿病患者的2倍。DM患者中性粒细胞经G-CSF或GM-CSF刺激后产生的H_2O_2水平较低，与未刺激的静息细胞无明显差异。这些数据表明，糖尿病患者由PMA诱导的中性粒细胞产生H_2O_2受到损害，而G-CSF和GM-CSF均不能促进其产生。
We evaluated the effects of granulocyte colony-stimulating factors (G-CSF), granulocyte-macrophage colony-stimulating factors (GM-CSF) and phorbol myristate acetate (PMA) on H2O2 production in purified neutrophils from patients with diabetes mellitus (DM), using flow cytometry. Twenty-two age-matched male subjects were selected: 11 were normal volunteers and the remainder had DM. No significant differences in intracellular H2O2 production per 60 min was observed in the 'resting' neutrophils from DM patients (37.2 +/- 20.4 A.U.) compared with those from normal volunteers (24.9 +/- 8.4 A.U.). The PMA-stimulated neutrophils from normal volunteers generated approximately 4-fold increases in H2O2 per 60 min compared with those from DM patients. Under similar culture conditions, G-CSF caused 1.6-fold increases of H2O2 in neutrophils from normal volunteers compared with those of DM patients. Increases after GM-CSF stimulation were 2-fold higher in volunteer neutrophils compared with those from DM patients. The levels of G-CSF- or GM-CSF-stimulated H2O2 production in neutrophils from DM patients were low and were little different from non-stimulated resting cells. These data showed that H2O2 production in neutrophils induced by PMA is impaired in patients with DM, and neither G-CSF nor GM-CSF enhances its production.