Enhanced proliferative potential in culture of cells from p53-deficient mice.
Enhanced proliferative potential in culture of cells from p53-deficient mice.
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p53 缺陷小鼠细胞培养物的增殖潜力增强。
作者：
Teruyo Tsukada;Yasuhiro Tomooka;Setsuo Takai;Yasuji Ueda;Shin;Takeshi Yagi;Tomoyuki Tokunaga;Naoki Takeda;Yoko Suda;Shinji Abe;Isao Matsuo;Yoji Ikawa;Shinichi Aizawa
正常的体细胞在培养中被赋予有限的倍增潜力，并且永生化过程是细胞的肿瘤转化中不可避免的步骤。为了研究p53在这一过程中的作用，研究了p53缺陷小鼠的细胞的倍增潜力。来自这些小鼠的各种组织的成纤维细胞样细胞持续增殖，而不显示衰老或危机。非整倍体细胞通过传代克服群体，但克隆实验表明，染色体变化不是这个过程所必需的。在透镜、乳腺和精囊上皮细胞以及神经前体细胞中，也观察到p53缺陷小鼠细胞培养物中的增殖潜力增强。骨髓细胞对干细胞因子的增殖反应在长期培养中增强，但在体外集落测定中没有增强;不能获得永久性细胞系。在心肌细胞或肝细胞的增殖中没有发现p53缺陷的影响。
Normal somatic cells are endowed with limited doubling potential in culture, and the process of immortalization is an inevitable step in neoplastic transformation of the cells. To examine the roles of p53 in this process, the cells of p53-deficient mice were examined for doubling potential. Fibroblast-like cells from a variety of tissues of these mice proliferated continuously without showing aging or crisis. The aneuploid cells overcome the population with passage, but cloning experiment indicated that chromosomal changes were not essential to this process. The enhanced proliferative potential in culture of cells from the p53-deficient mice was also observed in epithelial cells of lens, mammary glands and seminal vesicles and in neural precursor cells. Proliferation of bone marrow cells in response to stem cell factor was enhanced in long term culture, but not in in vitro colony assay; no permanent cell lines could be obtained. No effects of p53-deficiency were found in proliferation of cardiac muscle cells or hepatocytes.