Expression of an immunoglobulin heavy chain gene transfected into lymphocytes.
Expression of an immunoglobulin heavy chain gene transfected into lymphocytes.
复制标题
转染淋巴细胞的免疫球蛋白重链基因的表达。
DOI：
10.1073/pnas.81.5.1292
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发表时间：
1984
影响因子：
11.1
通讯作者：
Wall,R
中科院分区：
文献类型：
--
作者：
Deans,RJ;Denis,KA;Taylor,A;Wall,R
我们确定小鼠淋巴细胞系可以通过多瘤病毒穿梭载体以高效率（10-70%）转染。使用该载体，我们获得了转染到代表B淋巴细胞发育的所有阶段的细胞系、T细胞淋巴瘤系和3 T3成纤维细胞中的克隆小鼠α重链基因的表达。在转染的产生轻链的骨髓瘤细胞中，重链基因的表达水平与分泌IgA的骨髓瘤细胞相当。在转染的骨髓瘤细胞中产生的重链与膜结合伊加中的轻链相关。虽然T淋巴瘤细胞和成纤维细胞以与B细胞相似的效率转染，但产生显著较低水平的α重链。这种免疫球蛋白基因转染系统提供了一个强大的方法来定义免疫球蛋白基因的重要调控区域，并确定淋巴细胞因子参与免疫球蛋白基因表达的B淋巴细胞发育。
We determined that mouse lymphoid cell lines can be transfected at high efficiencies (10-70%) by a polyoma virus shuttle vector. With this vector, we obtained expression of a cloned mouse alpha heavy chain gene transfected into cell lines representative of all stages in B-lymphocyte development, a T-cell lymphoma line, and 3T3 fibroblasts. Heavy chain gene expression in transfected light chain-producing myeloma cells occurred at levels comparable to those in IgA-secreting myeloma cells. Heavy chains produced in transfected myeloma cells were associated with light chains in membrane-bound IgA. While T-lymphoma cells and fibroblasts were transfected at similar efficiencies to B cells, significantly lower levels of alpha heavy chains were produced. This immunoglobulin gene transfection system provides a powerful approach for defining important regulatory regions in immunoglobulin genes and for identifying lymphoid cell factors involved in immunoglobulin gene expression in B-lymphocyte development.