CXC chemokine receptor expression on human endothelial cells
CXC chemokine receptor expression on human endothelial cells
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DOI：
10.1006/cyto.1998.0465
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发表时间：
1999-09-01
期刊：
影响因子：
3.8
通讯作者：
Finn, A
中科院分区：
文献类型：
--
作者：
Murdoch, C;Monk, PN;Finn, A
CXC趋化因子在炎症部位的白细胞募集和激活过程中发挥重要作用。然而，最近的证据表明，这些分子也可以调节内皮细胞的功能，如迁移、血管生成和增殖。在本研究中，我们研究了CXC趋化因子受体在人脐静脉内皮细胞（HUVEC）和自发转化的HUVEC细胞系ECV304中的表达，我们发现两种细胞类型都表达趋化因子受体CXCR1、CXCR2和CXCR4的mRNA，但不表达CXCR3。流式细胞分析显示CXCR1表达水平较低，而CXCR4细胞表面表达水平较高。HUVECs对SDF-1 α的反应是快速而强大的钙通量，然而在IL-8或gro - α中没有观察到钙通量，HUVECs和ECV304细胞对CXC趋化因子没有增殖反应，尽管ECV304细胞确实向SDF-1 α和IL-8迁移，这些数据表明HUVECs和内皮细胞系ECV304表达功能性CXC趋化因子受体，(C) 1999学术出版社。
CXC Chemokines play a important role in the process of leukocyte recruitment and activation at sites of inflammation. However, recent evidence suggests that these molecules can also regulate endothelial cell functions such as migration, angiogenesis and proliferation, In this study we have investigated CXC chemokine receptor expression in both primary cultures of human umbilical vein endothelial cells (HUVEC) and the spontaneously transformed HUVEC cell line, ECV304, We found that both cell types express mRNA for chemokine receptors CXCR1, CXCR2 and CXCR4, but not CXCR3, Flow cytometric analysis revealed low levels of CXCR1 but higher levels of CXCR4 cell surface expression. HUVECs responded to SDF-1 alpha with a rapid and robust calcium flux, however no calcium flux was seen with either IL-8 or Gro-alpha, HUVECs and ECV304 cells did not proliferate in response to CXC chemokines, although ECV304 cells did migrate towards SDF-1 alpha and IL-8, These data demonstrate that HUVECs and the endothelial cell line, ECV304 express functional CXC chemokine receptors, (C) 1999 Academic Press.