Time-resolved observation of surface-enhanced Raman scattering from gold nanoparticles during transport through a living cell
Time-resolved observation of surface-enhanced Raman scattering from gold nanoparticles during transport through a living cell
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DOI：
10.1117/1.3119242
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发表时间：
2009-03-01
影响因子：
3.5
通讯作者：
Kawata, Satoshi
中科院分区：
文献类型：
--
作者：
Fujita, Katsumasa;Ishitobi, Sawako;Kawata, Satoshi
我们使用表面增强拉曼散射（Sers）对含有金纳米颗粒的活细胞进行时间分辨观察。利用狭缝扫描拉曼显微镜同时观测了50 nm金纳米粒子的位置和Sers光谱，具有较高的空间和时间分辨率。从Sers观察，我们证实了附着在细胞表面的颗粒和进入细胞与随后产生的Sers信号从附近的分子。我们还证实，强的依赖性的Sers光谱的粒子的位置在运输过程中的粒子通过细胞。所获得的Sers光谱及其时间波动表明，通过这种技术可观察到的分子信号仅从靠近纳米颗粒的有限体积内给出。这证实了Sers成像用于原位观察参与细胞事件的生物分子的高空间选择性和分辨率。(C)2009年，由光学仪器工程师协会（Society of Photo-Optical Instrumentation Engineers）主办。[DOI 10.1117/1.3119242]
We perform time-resolved observation of living cells with gold nanoparticles using surface-enhanced Raman scattering (SERS). The position and SERS spectra of 50-nm gold nanoparticles are simultaneously observed by slit-scanning Raman microscopy with high spatial and temporal resolution. From the SERS observation, we confirm the attachment of the particles on the cell surface and the entry into the cell with the subsequent generation of SERS signals from nearby molecules. We also confirm that the strong dependence of SERS spectra on the position of the particle during the transportation of the particle through the cell. The obtained SERS spectra and its temporal fluctuation indicate that the molecular signals observable by this technique are given only from within a limited volume in close proximity to the nanoparticles. This confirms the high spatial selectivity and resolution of SERS imaging for observation of biomolecules involved in cellular events in situ. (C) 2009 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3119242]