Glucose‐6‐phosphate isomerase from ehrlich ascites tumor cells: Purification and some properties
Glucose‐6‐phosphate isomerase from ehrlich ascites tumor cells: Purification and some properties
复制标题
艾氏腹水肿瘤细胞的葡萄糖-6-磷酸异构酶：纯化和一些特性
DOI：
10.1016/0014-5793(73)80166-8
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发表时间：
1973
期刊：
影响因子：
3.5
通讯作者：
F. Schneider
中科院分区：
文献类型：
--
作者：
K. Röhm;F. Schneider
Whereas extensive information is available on glucose-6-phosphate isomerases (EC 5.3. 1.9) purified from mammalian tissues such as skeletal muscle of rabbit [l], beef [2] and pig [2] as well as from baker’s [3] and brewer’s yeast [4] no attempt has been made so far to purify the enzyme from ascites tumor cells which are characterized by high glycolytic rates. Continuing previous experiments on the in vitro kinetics of glucosephosphate isomerase and application of the results to the calculation of glycolytic flux eg of the anaerobic yeast cells [5] we have attempted to isolate the enzyme from Ehrlich ascites tumor cells. The present paper describes these experiments; some properties of the purified preparation and kinetic parameters of the reaction catalyzed by the ascites tumor enzyme are also given.
