REGULATION OF ALLANTOATE TRANSPORT IN WILD-TYPE AND MUTANT STRAINS OF SACCHAROMYCES-CEREVISIAE
REGULATION OF ALLANTOATE TRANSPORT IN WILD-TYPE AND MUTANT STRAINS OF SACCHAROMYCES-CEREVISIAE
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DOI：
10.1128/jb.169.4.1684-1690.1987
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发表时间：
1987-04-01
影响因子：
3.2
通讯作者：
COOPER, TG
中科院分区：
文献类型：
--
作者：
CHISHOLM, VT;LEA, HZ;COOPER, TG
细胞内尿囊素和尿囊酸盐的积累是由酿酒酵母中两种不同的主动转运系统介导的。尿囊素转运（DAL4 基因）是诱导型的，而尿囊素吸收是组成型的（在培养基中不存在任何尿囊素相关化合物的情况下，尿囊素吸收以完全水平发生）。这两个系统似乎对氮分解代谢物抑制、反馈抑制和反式抑制敏感。缺乏尿囊素运输的突变体（dal5）已被分离出来。这些菌株还表现出 60% 的尿囊素转运能力损失。相反，之前描述的 dal4 突变体无法转运尿囊素，并且尿囊素转运损失 50%。我们将 dal4 和 dal5 突变的多效性行为解释为源自两个运输系统元件之间的功能相互作用。
Accumulation of intracellular allantoin and allantoate is mediated by two distinct active transport systems in Saccharomyces cerevisiae. Allantoin transport (DAL4 gene) is inducible, while allantoate uptake is constitutive (it occurs at full levels in the absence of any allantoate-related compounds from the culture medium). Both systems appear to be sensitive to nitrogen catabolite repression, feedback inhibition, and trans-inhibition. Mutants (dal5) that lack allantoate transport have been isolated. These strains also exhibit a 60% loss of allantoin transport capability. Conversely, dal4 mutants previously described are unable to transport allantoin and exhibit a 50% loss of allantoate transport. We interpret the pleiotropic behavior of the dal4 and dal5 mutations as deriving from a functional interaction between elements of the two transport systems.