Laboratory diagnosis in ulcerative keratitis - Experience in a tertiary care hospital
Laboratory diagnosis in ulcerative keratitis - Experience in a tertiary care hospital
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DOI：
10.1159/000084273
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发表时间：
2005-01-01
影响因子：
2.1
通讯作者：
Sethi, HS
中科院分区：
文献类型：
--
作者：
Khanal, B;Deb, M;Sethi, HS
目的：鉴定尼泊尔东部B.P.Koirala健康科学研究所(BPKIHS)常见的角膜溃疡细菌和真菌分离物，并确定细菌分离物对常用抗生素的药敏模式。并比较了培养与直接镜检的相关性和可靠性。方法：对1998年8月1日至2001年7月31日在北京协和医院眼科就诊的所有疑似角膜溃疡患者进行评估。角膜刮片用于直接显微镜检查和细菌和真菌分离培养。对分离的细菌进行药敏试验。结果：447例标本中，直接镜检阳性216例(48%)。179例(83%)标本的培养阳性与直接镜检结果相关。303例(67.8%)标本中有病原菌生长。其中单纯真菌145例(47.8%)，单纯细菌103例(34%)，真菌和细菌混合感染55例(18.2%)。最常见的真菌病原菌是曲霉属。78株(38.4%)，其次为镰刀菌属。45例(22%)。[医]金黄色葡萄球菌在25份(12.3%)标本中分离到一株。金黄色葡萄球菌(93，56.7%)是最常见的致病菌。肺炎链球菌(33株，占20%)位居第二。分离出的细菌对常用抗生素敏感。结论：本研究强调了持续监测药物及其药敏对预防和治疗角膜溃疡及其并发症的重要性和必要性。版权所有(C)2005 S.Karger AG，巴塞尔。
Aims: To identify the common bacterial and fungal isolates from corneal ulcers and to determine the antimicrobial susceptibility patterns of bacterial isolates to commonly used antibiotics at B. P. Koirala Institute of Health Sciences (BPKIHS), eastern Nepal. Culture and direct microscopic correlation and reliability were also compared. Methods: All patients with suspected corneal ulceration presenting to the Ophthalmology Department of BPKIHS from 1st August 1998 to 31st July 2001 were evaluated. Corneal scraping was performed and processed for direct microscopy and culture for bacterial and fungal isolates. Bacterial isolates were subjected to antimicrobial susceptibility testing. Results: Of 447 specimens examined direct microscopy was positive in 216 (48%) specimens. Culture positivity could be correlated with direct microscopy in 179 (83%) of specimens. Growth of etiologic agents was found in 303 (67.8%) samples. Of these 145 (47.8%) had pure fungal growth, 103 (34%) had pure bacterial growth and 55 (18.2%) had mixed fungal and bacterial infection. The commonest fungal pathogen was Aspergillus spp. in 78 (38.4%) followed by Fusarium spp. in 45 (22%). Aureobasidium sp. was isolated in 25 (12.3%) samples. Staphylococcus aureus (93, 56.7%) dominated the scene as the commonest bacterial agent. Streptococcus pneumoniae (33, 20%) was second in the list. Most of the bacterial isolates were sensitive to commonly used antibiotics. Conclusion: This study emphasizes the importance and need of the continued surveillance of the agents and their antimicrobial susceptibility for the prevention and management of corneal ulcers and their complications. Copyright (C) 2005 S. Karger AG, Basel.