High phosphate diet suppresses lipogenesis in white adipose tissue.
High phosphate diet suppresses lipogenesis in white adipose tissue.
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DOI：
10.3164/jcbn.17-141
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发表时间：
2018-11
影响因子：
2.4
通讯作者：
Taketani Y
中科院分区：
文献类型：
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作者：
Imi Y;Yabiki N;Abuduli M;Masuda M;Yamanaka-Okumura H;Taketani Y
过量的磷酸盐摄入与肾脏和血管功能障碍呈正相关，与体脂积累呈负相关。我们研究了高磷饮食对白色脂肪组织和肝脏中脂代谢基因表达的影响。8周龄雄性SD大鼠饲喂含0.6%磷酸盐的对照饲料或含1.5%磷酸盐的高磷饲料4周。与对照组相比，HP组的体脂质量和空腹血浆胰岛素水平显著降低，内脏脂肪中脂肪生成减少，脂解基因表达增加。此外，高磷组涉及肝脏脂肪生成、肝脏糖生成和甘油三酯积累的基因表达减少。外源性磷酸盐、甲状旁腺激素和成纤维细胞生长因子23不直接影响3T3-L1脂肪细胞和HepG2肝细胞的脂解或成脂基因的表达。因此，高磷饮食通过增加脂肪分解基因的表达和降低成脂基因的表达来抑制白色脂肪组织的活性。这些影响可能是由高磷饮食引起的空腹血浆胰岛素水平降低造成的，但不是由血浆磷或磷酸尿激素的增加直接引起的。
Excessive phosphate intake has been positively associated with renal and vascular dysfunction, conversely negatively associated with body fat accumulation. We investigated the effect of a high-phosphate diet on the expression of lipid metabolic genes in white adipose tissue and liver. Male 8-week-old Sprague–Dawley rats were fed a control diet containing 0.6% phosphate or a high-phosphate diet containing 1.5% phosphate for 4 weeks. In comparison to the control group, the HP group showed a significantly lower body fat mass and fasting plasma insulin level alongside decreased lipogenic and increased lipolytic gene expression in visceral fat. Additionally, the expression of genes involved in hepatic lipogenesis, hepatic glycogenesis, and triglyceride accumulation decreased in the high-phosphate group. Exogenous phosphate, parathyroid hormone, and fibroblast growth factor 23 did not directly affect the expression of lipolytic or lipogenic genes in 3T3-L1 adipocytes and HepG2 hepatocytes. Thus, the high-phosphate diet suppressed the activity of white adipose tissue by increasing lipolytic gene expression and decreasing lipogenic gene expression. These effects could have been caused by the lowered fasting plasma insulin level that occurred in response to the high-phosphate diet, but were not directly caused by the increases in plasma phosphate or phosphaturic hormones.