Effects of detergents on Ca(2+)-activated neural proteinase activity (calpain) in neural and non-neural tissue: a comparative study.
Effects of detergents on Ca(2+)-activated neural proteinase activity (calpain) in neural and non-neural tissue: a comparative study.
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去污剂对神经和非神经组织中 Ca(2) 激活的神经蛋白酶活性（钙蛋白酶）的影响：一项比较研究。
DOI：
10.1007/bf00969015
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发表时间：
1992
影响因子：
4.4
通讯作者：
Hogan,EL
中科院分区：
文献类型：
--
作者：
Banik,NL;Chakrabarti,AK;Hogan,EL
测定了大鼠脑组织和其他组织中钙活化中性蛋白酶（mcalpain）的活性。超过60%的脑钙蛋白酶活性存在于颗粒部分，而只有30%存在于细胞质中。相比之下，肝脏、肾脏、肌肉和心脏的颗粒组分含有约8-12%的组织肌痛活性，而细胞质中存在88%的组织肌痛活性。去除内源性抑制剂calpastatin后，组织蛋白酶活性增加了数倍。Triton X-100和脱氧胆酸盐（DOC）刺激神经钙蛋白酶活性10倍，而非神经组织的活性不受影响。与其他洗涤剂（如Triton N-57和硫代糖苷）孵育后，脑钙蛋白酶活性刺激了5倍，而Brij-35没有任何影响。另一方面，十二烷基硫酸钠（SDS）抑制酶的活性。与非神经组织相比，大脑的钙蛋白酶活性最低。肌肉、肾脏和心脏的钙蛋白酶活性是肝脏的3倍。免疫印迹分析表明，钙蛋白酶主要存在于脑组织的颗粒部分，胞质中含量较少；而在非神经组织的颗粒部分，钙蛋白酶主要存在于胞质中，含量较少。
Calcium activated neutral proteinase (mcalpain) activity was determined in brain and other tissue of rat. More than 60% of the brain mcalpain activity was present in the particulate fraction while only 30% was in cytosol. In contrast, particulate fractions of liver, kidney, muscle, and heart contained about 8–12% of tissue mcalpain activity while 88% was present in cytosol. Removal of the endogenous inhibitor calpastatin increased the tissue mcalpain activity severalfold. Triton X-100 and deoxycholate (DOC) stimulated the neural calpain activity by ten-fold while activity in non-neural tissue was unaffected. Incubation with other detergents, e.g. Triton N-57 and thioglucopyranoside, stimulated brain calpain activity five-fold while Brij-35 did not have any effect. Sodiumdodecylsulphate (SDS), on the other hand, inhibited the enzyme activity. Brain contained the lowest calpain activity compared to non-neural tissue. The calpain activity in muscle, kidney and heart was three-fold greater than liver. Immunoblot identification of the enzyme revealed that calpain was predominantly in the particulate fraction and less in cytosol of brain while it was present mainly in cytosol and less in the pellet fractions of non-neural tissue.