Model suggesting that replication of influenza virus is regulated by stabilization of replicative intermediates
Model suggesting that replication of influenza virus is regulated by stabilization of replicative intermediates
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DOI：
10.1128/jvi.78.17.9568-9572.2004
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发表时间：
2004-09-01
影响因子：
5.4
通讯作者：
Brownlee, GG
中科院分区：
文献类型：
--
作者：
Vreede, FT;Jung, TE;Brownlee, GG
甲型流感病毒的RNA依赖性RNA聚合酶负责负义病毒RNA的转录和复制。据认为，“转换”机制调节这些活动之间的过渡。我们证明，在预先存在的病毒RNA聚合酶和核蛋白（NP）的存在下，甲型流感病毒在感染早期合成mRNA（转录）和cRNA（复制）。我们认为，可能没有开关调节RNA合成的起始，并提出了一个模型，表明新生的cRNA被宿主细胞核酸酶降解，除非它是稳定的新合成的病毒RNA聚合酶和NP。
The RNA-dependent RNA polymerase of influenza A virus is responsible for both transcription and replication of negative-sense viral RNA. It is thought that a "switching" mechanism regulates the transition between these activities. We demonstrate that, in the presence of preexisting viral RNA polymerase and nucleoprotein (NP), influenza A virus synthesizes both mRNA (transcription) and cRNA (replication) early in infection. We suggest that there may be no switch regulating the initiation of RNA synthesis and present a model suggesting that nascent cRNA is degraded by host cell nucleases unless it is stabilized by newly synthesized viral RNA polymerase and NP.