Muscle net glucose uptake and glucose kinetics after endurance training in men
Muscle net glucose uptake and glucose kinetics after endurance training in men
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DOI：
10.1152/ajpendo.1999.277.1.e81
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发表时间：
1999-07-01
影响因子：
5.1
通讯作者：
Brooks, GA
中科院分区：
文献类型：
--
作者：
Bergman, BC;Butterfield, GE;Brooks, GA
我们评估了这样的假设：耐力训练引起的葡萄糖处理率（R-d）的变化是活动肌肉净葡萄糖吸收变化的结果，并且在需要高相对功率输出的运动过程中血糖被分流到工作肌肉。我们研究了训练前 [45% 和 65% 的耗氧峰值速率 ((V) 超过点 o(2peak))] 和训练后 [65% 训练前 (V) 超过点 o(2peak)，相同的绝对工作量 (ABT)，以及 65% 训练后 (V) 超过点 o(2peak)，相同的相对工作量，两种强度的 1 小时自行车测力期间的腿部净葡萄糖摄取量 （RLT）]。对 9 名男性受试者（178.1 +/- 2.5 厘米、81.8 +/- 3.3 公斤、27.4 +/- 2.0 岁）在为期 9 周的自行车测力计训练之前和之后进行了测试，每周 5 次，测试频率为 75% (V) 超过 dot o(2peak)。训练前在点 o(2peak) 上引起 66.0 +/- 1.1% (V) 的功率输出在训练后引起 54.0 +/- 1.7%。训练后，ABT 时全身葡萄糖 R-d 下降（65% 训练前为 5.45 +/- 0.31 mg . kg(-1) . min(-1)，降至 4.36 +/- 0.44 mg . kg(-1) . min(-1)），但 RLT 时则没有下降（5.94 +/- 0.47 mg . kg(-1) . min(-1)）。训练后 ABT 时的净葡萄糖摄取量有所减弱（65% 训练前为 1.87 +/- 0.42 mmol/min，训练前为 0.54 +/- 0.33 mmol/min），但 RLT 时则没有（2.25 +/- 0.81 mmol/min）。 ABT 时腿部净葡萄糖摄取的减少与葡萄糖 Rd 的下降幅度相似，因此可以解释训练后葡萄糖通量的减弱。糖原降解也会减少 ABT 训练后的情况，但不会减少 RLT 的情况。腿部净葡萄糖摄取占训练前血糖流的 61%，在相同相对工作量（65% (V) over dot o(2peak)）训练后占 81%，而在 ABT 训练后仅占 38%。我们的结论是：1）训练时主动肌肉葡萄糖摄取的变化决定了全身葡萄糖动力学的变化； 2) 训练后，对于给定的中等强度任务，肌肉葡萄糖摄取减少； 3) 剧烈运动（65% (V) over dot o(2peak)）促进葡萄糖从不活跃的组织分流到活跃的肌肉。
We evaluated the hypotheses that alterations in glucose disposal rate (R-d) due to endurance training are the result of changed net glucose uptake by active muscle and that blood glucose is shunted to working muscle during exercise requiring high relative power output. We studied leg net glucose uptake during 1 h of cycle ergometry at two intensities before training [45 and 65% of peak rate of oxygen consumption ((V) over dot o(2peak))] and after training [65% pretraining (V) over dot o(2peak), same absolute workload (ABT), and 65% posttraining (V) over dot o(2peak), same relative workload (RLT)]. Nine male subjects (178.1 +/- 2.5 cm, 81.8 +/- 3.3 kg, 27.4 +/- 2.0 yr) were tested before and after 9 wk of cycle ergometer training, five times a week at 75% (V) over dot o(2peak). The power output that elicited 66.0 +/- 1.1% of (V) over dot o(2peak) before training elicited 54.0 +/- 1.7% after training. Whole body glucose R-d decreased posttraining at ABT (5.45 +/- 0.31 mg . kg(-1) . min(-1) at 65% pretraining to 4.36 +/- 0.44 mg . kg(-1) . min(-1)) but not at RLT (5.94 +/- 0.47 mg . kg(-1) . min(-1)). Net glucose uptake was attenuated posttraining at ABT (1.87 +/- 0.42 mmol/min at 65% pretraining and 0.54 +/- 0.33 mmol/min) but not at RLT (2.25 +/- 0.81 mmol/min). The decrease in leg net glucose uptake at ABT was of similar magnitude as the drop in glucose Rd and thus could explain dampened glucose flux after training. Glycogen degradation also decreased posttraining at ABT but not RLT. Leg net glucose uptake accounted for 61% of blood glucose flux before training and 81% after training at the same relative (65% (V) over dot o(2peak)) workload and only 38% after training at ABT. We conclude that 1) alterations in active muscle glucose uptake with training determine changes in whole body glucose kinetics; 2) muscle glucose uptake decreases for a given, moderate intensity task after training; and 3) hard exercise (65% (V) over dot o(2peak)) promotes a glucose shunt from inactive tissues to active muscle.