Integrated nanopore/nicrochannel devices for ac electroldnetic trapping of particles
Integrated nanopore/nicrochannel devices for ac electroldnetic trapping of particles
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DOI：
10.1021/ac701759f
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发表时间：
2008-02-01
影响因子：
7.4
通讯作者：
Jacobson, Stephen C.
中科院分区：
文献类型：
--
作者：
Kovarik, Michelle L.;Jacobson, Stephen C.
我们报告了集成的纳米孔/微流体装置，其中低孔密度、锥形纳米孔膜与微流体通道的独特组合创建了用于电泳和介电泳捕获颗粒的可寻址的局部高场区域。一个聚（对苯二甲酸乙二醇酯）的轨道蚀刻膜包含圆锥形孔类似于130 nm的直径在尖端和类似于1 μ m的直径在基地被用作两个垂直的聚（二甲基硅氧烷）微流体通道之间的互连。集成的纳米孔膜与微流体通道允许容易耦合的电势和直接运输的分析物颗粒，200 nm和1 μ m的聚苯乙烯微球和新月柄杆菌细菌，捕获区域。应用于该装置的方波在微通道交叉点的纳米孔尖端产生高达1.3 × 10（5）V/cm的电场强度。通过改变外加电位从+/- 10到+/- 100 V和探索频率从直流到100 kHz，我们确定了电泳和介电泳力的捕获和浓缩过程的贡献。这些结果表明，可调谐滤波器元件可以构建其中themanoporous元件提供了一个物理屏障和所施加的交流电场增强的选择性。
We report integrated nanopore/microfluidic devices in which the unique combination of low pore density, conical nanopore membranes with microfluidic channels created addressable, localized high-field regions for electrophoretic and dielectrophoretic trapping of particles. A poly(ethylene terephthalate) track-etched membrane containing conical pores similar to 130 nm in diameter at the tip and similar to 1 mu m in diameter at the base was used as an interconnect between two perpendicular poly(dimethylsiloxane) microfluidic channels. Integration of the nanopore membrane with microfluidic channels allowed for easy coupling of the electrical potentials and for directed transport of the analyte particles, 200 nm and 1 mu m polystyrene microspheres and Caulobacter crescentus bacteria, to the trapping region. Square waves applied to the device generated electric field strengths up to 1.3 x 10(5) V/cm at the tips of the nanopores in the microchannel intersection. By varying the applied potentials from +/- 10 to +/- 100 V and exploring frequencies from dc to 100 kHz, we determined the contributions of electrophoretic and dielectrophoretic forces to the trapping and concentration process. These results suggest that tunable filter elements can be constructed in which themanoporous elements provide a physical barrier and the applied ac field enhanced selectivity.