Imaging hypoxia in orthotopic rat liver tumors with iodine 124-labeled iodoazomycin galactopyranoside PET
Imaging hypoxia in orthotopic rat liver tumors with iodine 124-labeled iodoazomycin galactopyranoside PET
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DOI：
10.1148/radiol.2482071421
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发表时间：
2008-08-01
期刊：
影响因子：
19.7
通讯作者：
Fong, Yuman
中科院分区：
文献类型：
--
作者：
Riedl, Christopher C.;Brader, Peter;Fong, Yuman
目的：通过将高 I-124-IAZGP 摄取区域与独立的缺氧测量进行比较，评估碘 124 (I-124) 标记的碘阿霉素吡喃半乳糖苷 (IAZGP) 正电子发射断层扫描 (PET) 在原位大鼠肝脏肿瘤模型中检测缺氧的效果，并确定注射后描述缺氧的最佳时间。 材料和方法：机构动物护理和使用委员会批准了这项研究。在 15 只大鼠的肝脏中建立莫里斯肝癌肿瘤。使用荧光光纤氧探针测量两只大鼠的肿瘤氧合。 I-124-IAZGP 与已建立的缺氧标志物哌莫硝唑和 EF5 共同给予 9 只大鼠； 24小时后进行12小时PET数据采集。用免疫荧光和放射自显影分析肿瘤冷冻切片。在其余四只大鼠中，在示踪剂施用后长达 48 小时内进行连续 20 分钟和 60 分钟 PET 数据采集。结果：氧探针测量显示严重缺氧（
Purpose: To evaluate iodine 124 (I-124)-labeled iodoazomycin galactopyranoside (IAZGP) positron emission tomography (PET) in the detection of hypoxia in an orthotopic rat liver tumor model by comparing regions of high I-124-IAZGP uptake with independent measures of hypoxia and to determine the optimal time after injection to depict hypoxia.Materials and Methods: The institutional animal care and use committee approved this study. Morris hepatoma tumors were established in the livers of 15 rats. Tumor oxygenation was measured in two rats with a fluorescence fiberoptic oxygen probe. I-124-IAZGP was coadministered with the established hypoxia markers pimonidazole and EF5 in nine rats; 12-hour PET data acquisition was performed 24 hours later. Tumor cryosections were analyzed with immunofluorescence and autoradiography. In the four remaining rats, serial 20- and 60-minute PET data acquisition was peformed up to 48 hours after tracer administration.Results: Oxygen probe measurements showed severe hypoxia (