FDISCO: Advanced solvent-based clearing method for imaging whole organs
FDISCO: Advanced solvent-based clearing method for imaging whole organs
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FDISCO：用于整个器官成像的先进溶剂型透明化方法
DOI：
10.1126/sciadv.aau8355
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发表时间：
2019-01-01
期刊：
影响因子：
13.6
通讯作者：
Zhu, Dan
中科院分区：
文献类型：
--
作者：
Qi, Yisong;Yu, Tingting;Zhu, Dan
各种光学透明方法已成为深层生物成像的有力工具。基于有机溶剂的透明方法，例如溶剂透明器官的三维成像（3DISCO），具有高透明效率以及能对诸如整个器官甚至整个身体等大样本进行全景成像时缩小尺寸的优势。然而，3DISCO会导致内源性荧光迅速猝灭，这阻碍了它的应用。在此，我们提出一种名为FDISCO的先进方法来克服这一局限。FDISCO能够有效保留各种荧光探针的荧光，并且在保持强大透明能力的同时可实现长达数月的储存时间。我们利用FDISCO对神经元和血管网络进行高分辨率成像和重建。此外，FDISCO与多种病毒标记兼容，并能够对全脑中荧光标记较弱的神经元进行精细可视化。FDISCO是对整个器官进行三维成像的一种有效替代方法，可广泛应用于生物医学研究。
Various optical clearing methods have emerged as powerful tools for deep biological imaging. Organic solvent-based clearing methods, such as three-dimensional imaging of solvent-cleared organs (3DISCO), present the advantages of high clearing efficiency and size reduction for panoptic imaging of large samples such as whole organs and even whole bodies. However, 3DISCO results in a rapid quenching of endogenous fluorescence, which has impeded its application. Here, we propose an advanced method named FDISCO to overcome this limitation. FDISCO can effectively preserve the fluorescence of various fluorescent probes and can achieve a long storage time of months while retaining potent clearing capability. We used FDISCO for high-resolution imaging and reconstruction of neuronal and vascular networks. Moreover, FDISCO is compatible with labeling by multiple viruses and enables fine visualization of neurons with weak fluorescence labeling in the whole brain. FDISCO represents an effective alternative to the three-dimensional mapping of whole organs and can be extensively used in biomedical studies.