Highly Efficient Intramolecular Electrochemiluminescence Energy Transfer for Ultrasensitive Bioanalysis of Aflatoxin M1.
Highly Efficient Intramolecular Electrochemiluminescence Energy Transfer for Ultrasensitive Bioanalysis of Aflatoxin M1.
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DOI：
10.1002/chem.201604411
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发表时间：
2017-02
期刊：
影响因子：
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通讯作者：
Jia-Li Liu;Min Zhao;Ying Zhuo;Y. Chai;R. Yuan
中科院分区：
文献类型：
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作者：
Jia-Li Liu;Min Zhao;Ying Zhuo;Y. Chai;R. Yuan
鲁米诺与Ru（bpy）32+之间的分子间电化学发光共振能量转移（ECL-RET）因供体和受体的完美光谱重叠而被广泛研究，以实现灵敏的生物分析，但仍然存在能量转移效率低的挑战性问题。设计并研究了以鲁米诺为给体、Ru（bpy）2（mcpbpy）2+为受体的新型电化学发光化合物（Lum-Ru）的分子内电化学发光-共振电子转移（ECL-RET）。由于鲁米诺与Ru（bpy）2（mcpbpy）2+之间的能量传输路径短，能量损失小，因此在一个分子中实现了高效的ECL-RET，从而获得了高强度的Lum-Ru电化学发光信号。进一步应用Lum-Ru构建了一种信号关闭电化学发光（ECL）适体传感器，用于检测强致癌物黄曲霉毒素M1（AFM 1）。该传感平台还为临床分析中的其他生物分子的痕量检测提供了显着的提升。
The intermolecular electrochemiluminescence resonance energy transfer (ECL-RET) between luminol and Ru(bpy)32+ was studied extensively to achieve the sensitive bioanalysis owing to the perfect spectral overlap of the donor and acceptor, but it still suffers from the challenging issue of low energy-transfer efficiency. The intramolecular ECL-RET towards the novel ECL compound containing the donor of luminol and the acceptor of Ru(bpy)2 (mcpbpy)2+ (Lum-Ru) was designed and investigated. With the high-efficient ECL-RET in one molecule, the highly intense ECL signal of Lum-Ru was obtained owing to the short path of energy transmission and less energy loss between luminol and Ru(bpy)2 (mcpbpy)2+ . Lum-Ru was further applied to construct a signal-off electrochemiluminescence (ECL) aptasensor for ultrasensitive detection of a harsh carcinogen of Aflatoxin M1 (AFM1). This sensing platform also provides a significant boost for the trace detection of other biomolecules in clinical analysis.