Pantoea coffeiphila sp nov., cause of the 'potato taste' of Arabica coffee from the African Great Lakes region

Pantoea coffeiphila sp nov., cause of the 'potato taste' of Arabica coffee from the African Great Lakes region
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DOI:
10.1099/ijs.0.063545-0
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发表时间:
2015-01-01
影响因子:
2.8
通讯作者:
Cilas, Christian
Cilas, Christian
中科院分区:
生物学3区
文献类型:
--
作者:
Gueule, Dominique;Fourny, Gerard;Cilas, Christian

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研究了从咖啡种子中分离出的6株具有马铃薯风味的菌株。基因序列分析(rrs和rpoB)表明它们属于泛菌属。通过DNA-DNA杂交,分离物构成了一个基因组种,与96株代表肠杆菌种的菌株的亲缘性小于17%。多位点序列分析(gyrB、rpoB、atpD和infB基因)表明,这些分离物代表了Pantoea属的一个独立种。新种的营养多样性较差。该新种为Pantoea coffeiphila sp. nov.,其类型菌株为Ca04(T) (=CIP 110718(T)=DSM 28482(T))。
Six isolates recovered from coffee seeds giving off a potato-like flavour were studied. Gene sequencing (rrs and rpoB) showed they belong to the genus Pantoea. By DNA-DNA hybridization, the isolates constituted a genomic species with less than 17% relatedness to 96 strains representing enterobacterial species. Multilocus sequence analysis (gyrB, rpoB, atpD and infB genes) showed the isolates to represent a discrete species of the genus Pantoea. Nutritional versatility of the novel species was poor. The novel species is proposed as Pantoea coffeiphila sp. nov. and its type strain is Ca04(T) (=CIP 110718(T)=DSM 28482(T)).