Construction of a Binary Vector for Transformation of Arabidopsis thaliana with a New Selection Marker
Construction of a Binary Vector for Transformation of Arabidopsis thaliana with a New Selection Marker
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DOI：
10.1271/bbb.80428
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发表时间：
2008-11
期刊：
影响因子：
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通讯作者：
N. Koizumi;Yuji Iwata
中科院分区：
文献类型：
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作者：
N. Koizumi;Yuji Iwata
构建了一个新的二元载体 pZT4B，其中包含 UDP-N-乙酰氨基葡萄糖：多甘醇磷酸 N-乙酰氨基葡萄糖-1-P 转移酶 (GPT) 基因作为选择标记。将绿色荧光蛋白(GFP)基因插入pZT4B中，并将所得质粒用于拟南芥的转化。用0.3mg/l衣霉素筛选后获得的所有6个独立转化体均含有转基因并显示GFP荧光。
A new binary vector, pZT4B, containing the UDP-N-acetylglucosamine: dolichol phosphate N-acetylglucosamine-1-P transferase (GPT) gene as a selection marker, was constructed. The green fluorescent protein (GFP) gene was inserted into pZT4B, and the resulting plasmid was used in the transformation of Arabidopsis. All of six independent transformants obtained after selection with 0.3 mg/l tunicamycin contained the transgene and showed GFP fluorescence.