Human immunodeficiency virus type 1 derivative with 7% simian immunodeficiency virus genetic content is able to establish infections in pig-tailed macaques
Human immunodeficiency virus type 1 derivative with 7% simian immunodeficiency virus genetic content is able to establish infections in pig-tailed macaques
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DOI：
10.1128/jvi.00960-07
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发表时间：
2007-10-01
影响因子：
5.4
通讯作者：
Martin, Malcolm A.
中科院分区：
文献类型：
--
作者：
Igarashi, Tatsuhiko;Iyengar, Ranjini;Martin, Malcolm A.
人类免疫缺陷病毒 1 型 (HIV-1) 衍生物 (HIVNL-DT5R) 含有编码 CA 7 个氨基酸片段的序列和来自猿猴免疫缺陷病毒 (SIV) 的完整 vif 基因，此前已被证明可在培养的猕猴外周血单核细胞中建立传播感染。为了评估其体内复制和疾病诱导特性，将 HIVNL-DT5R 接种到猪尾猕猴体内。 HIVNL-DT5R在所有五只猴子中产生血浆病毒血症，并引发针对所有HfV-1结构蛋白的体液反应，但不会引起CD4(+) T淋巴细胞耗竭或临床疾病。需要额外的适应来优化体内感染性。
A human immunodeficiency virus type 1 (HIV-1) derivative (HIVNL-DT5R) containing sequences encoding a 7-amino-acid segment of CA and the entire vif gene from simian immunodeficiency virus (SIV) was previously shown to establish spreading infections in cultured macaque peripheral blood mononuclear cells. To assess its replicative and disease-inducing properties in vivo, HIVNL-DT5R was inoculated into pig-tailed macaques. HIVNL-DT5R generated plasma viremia in all five of the monkeys and elicited humoral responses against all of the HfV-1 structural proteins but did not cause CD4(+) T-lymphocyte depletion or clinical disease. Additional adaptation will be required to optimize infectivity in vivo.