Dimers of mitochondrial ATP synthase induce membrane curvature and self-assemble into rows
Dimers of mitochondrial ATP synthase induce membrane curvature and self-assemble into rows
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DOI：
10.1073/pnas.1816556116
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发表时间：
2019-03-05
影响因子：
11.1
通讯作者：
Kuehlbrandt, Werner
中科院分区：
文献类型：
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作者：
Blum, Thorsten B.;Hahn, Alexander;Kuehlbrandt, Werner
线粒体ATP酶形成二聚体，在内膜嵴边缘组装成长带。我们重建洗涤剂纯化的线粒体ATP合酶二聚体从绿色藻类Polytomella sp.和酵母Yarrowia lipolytica到脂质体，并检查他们的电子冷冻断层扫描。层析体积显示，ATP合酶二聚体从两个物种自组装成行和弯曲局部的脂质双层。二聚体行和诱导的膜弯曲程度与内膜嵴非常相似。线粒体ATP合酶的单体重组到脂质体中不明显地使膜弯曲，也不形成行。除了ATP合酶二聚体之外，不需要特定的脂质或蛋白质来进行行形成和膜重塑。ATP合酶二聚体的长排是线粒体内膜的保守特征。它们是嵴形成所必需的，也是线粒体形态发生的主要因素。
Mitochondrial ATP synthases form dimers, which assemble into long ribbons at the rims of the inner membrane cristae. We reconstituted detergent-purified mitochondrial ATP synthase dimers from the green algae Polytomella sp. and the yeast Yarrowia lipolytica into liposomes and examined them by electron cryotomography. Tomographic volumes revealed that ATP synthase dimers from both species self-assemble into rows and bend the lipid bilayer locally. The dimer rows and the induced degree of membrane curvature closely resemble those in the inner membrane cristae. Monomers of mitochondrial ATP synthase reconstituted into liposomes do not bend membrane visibly and do not form rows. No specific lipids or proteins other than ATP synthase dimers are required for row formation and membrane remodelling. Long rows of ATP synthase dimers are a conserved feature of mitochondrial inner membranes. They are required for cristae formation and a main factor in mitochondrial morphogenesis.