Determination of endogenous peptides in the porcine brain: possible construction of Peptidome, a fact database for endogenous peptides
Determination of endogenous peptides in the porcine brain: possible construction of Peptidome, a fact database for endogenous peptides
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DOI：
10.1016/s1570-0232(03)00280-0
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发表时间：
2003-07-15
影响因子：
3
通讯作者：
Takao, T
中科院分区：
文献类型：
--
作者：
Minamino, N;Tanaka, J;Takao, T
肽在许多生理事件中起着至关重要的作用。然而，内源性肽的数据库尚未开发，因为肽在提取和纯化过程中容易被蛋白水解酶降解。在这项研究中，我们证明了内源性肽的数据可以通过最小化蛋白水解降解来收集。我们分离猪脑肽成5250级分的二维色谱法（第一离子交换和第二反相高效液相色谱法），75级分的平均肽含量进行了详细的质谱仪和蛋白质测序仪分析。基于本研究中获得的分析数据，从2g脑组织开始，推断出超过10 000个肽被检测，并且超过1000个肽被鉴定。因此，我们推断，它是可能的，以构建一个数据库的内源性肽。从克级的组织开始，通过使用二维高。高效液相色谱-质谱联用。(C)2003 Elsevier Science B. V.保留所有权利。
Peptides play crucial roles in many physiological events. However, a database for endogenous peptides has not yet been developed, because the peptides are easily degraded by proteolytic enzymes during extraction and purification. In this study, we demonstrated that the data for endogenous peptides could be collected by minimizing the proteolytic degradation. We separated porcine brain peptides into 5250 fractions by 2-dimensional chromatography (first ion-exchange and second reversed-phase high-performance, liquid chromatography), and 75 fractions of average peptide contents were analyzed in detail by mass spectrometers and a protein sequencer. Based on the analysis data obtained in this study, more than 10 000 peptides were deduced to be detected, and more than 1000 peptides to be identified starting from 2 g of brain tissue. Thus, we deduce that it is possible to construct a database for endogenous peptides. starting from a gram level of tissue by using 2-dimensional high.-Performance liquid chromatography coupled with a mass spectrometer. (C) 2003 Elsevier Science B.V. All rights reserved.