Effect of UVA-activated Riboflavin on Dentin Bonding
Effect of UVA-activated Riboflavin on Dentin Bonding
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DOI：
10.1177/0022034511423397
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发表时间：
2011-12-01
影响因子：
7.6
通讯作者：
Mazzoni, A.
中科院分区：
文献类型：
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作者：
Cova, A.;Breschi, L.;Mazzoni, A.
最近的研究报告了暴露于核黄素后，接着暴露于紫外线A（UVA）后的胶原交联。这项研究是第一次调查的影响，核黄素含有底漆的粘接界面的稳定性和牙本质基质金属蛋白酶的活性。人牙本质用35%磷酸蚀刻，用0.1%核黄素处理，暴露于UVA 2分钟，并用两步蚀刻和冲洗粘合剂粘合。将粘合剂应用于不含核黄素/UVA的对照样本。对样本进行微拉伸粘合强度测试，并在储存24小时、6个月或1年后拉伸至失效。通过光学和透射电子显微镜评价界面纳米泄漏。为了研究牙本质基质金属蛋白酶的活性，我们进行了相关的酶谱分析，从磷酸蚀刻牙本质粉与核黄素/UVA处理或不处理和XP债券的蛋白提取物。紫外线激活的核黄素治疗增加了所有老化间隔的牙本质的即时粘结强度（p < 0.05 vs.对照组），并减少了老化样本的界面纳米渗漏（1年; p < 0.05）。酶谱显示核黄素/UVA预处理抑制牙本质基质金属蛋白酶活性（尤其是MMP-9）。总之，核黄素/UVA诱导的牙本质胶原交联增加了即刻粘结强度，稳定了粘结界面，并抑制了牙本质基质金属蛋白酶，从而增加了树脂-牙本质粘结的耐久性。
Recent studies have reported collagen cross-linking after exposure to riboflavin followed by ultraviolet-A (UVA) exposure. This study is the first to investigate the effect of a riboflavin-containing primer on adhesive interface stability and dentinal matrix metalloproteinase activity. Human dentin was etched with 35% phosphoric acid, treated with 0.1% riboflavin, exposed to UVA for 2 min, and bonded with a two-step etch-and-rinse adhesive. Adhesive was applied to control specimens without riboflavin/UVA. Specimens were subjected to microtensile bond strength tests and pulled to failure after storage for 24 hrs, 6 mos, or 1 yr. Interfacial nanoleakage was evaluated by light and transmission electron microscopy. To investigate dentinal matrix metalloproteinase activity, we performed correlative zymographic assays on protein extracts obtained from phosphoric-acid-etched dentin powder with or without riboflavin/UVA treatment and XP Bond. Ultraviolet-activated riboflavin treatment increased the immediate bond strength to dentin at all aging intervals (p < 0.05 vs. control) and decreased interfacial nanoleakage in aged specimens (1 yr; p < 0.05). Zymograms revealed that riboflavin/UVA pre-treatment inhibited dentinal matrix metalloproteinase activity (especially MMP-9). In conclusion, dentinal collagen cross-linking induced by riboflavin/UVA increased immediate bond strength, stabilized the adhesive interface, and inhibited dentin matrix metalloproteinases, thereby increasing the durability of resin-dentin bonds.