A PANEL APPROACH TO THE EVALUATION OF THE SENSITIVITY AND SPECIFICITY OF ANTIBODIES FOR THE DIAGNOSIS OF ROUTINELY PROCESSED HISTOLOGICALLY UNDIFFERENTIATED HUMAN NEOPLASMS
A PANEL APPROACH TO THE EVALUATION OF THE SENSITIVITY AND SPECIFICITY OF ANTIBODIES FOR THE DIAGNOSIS OF ROUTINELY PROCESSED HISTOLOGICALLY UNDIFFERENTIATED HUMAN NEOPLASMS
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DOI：
10.1093/ajcp/88.4.457
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发表时间：
1987-10-01
影响因子：
3.5
通讯作者：
ROUSE, RV
中科院分区：
文献类型：
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作者：
MICHIE, SA;SPAGNOLO, DV;ROUSE, RV
在组织学上无法诊断的肿瘤的常规处理切片上评价用于诊断目的的抗体存在特殊问题。作者通过构建一组puerectin相互排斥的抗体并在间变性肿瘤切片上进行测试来解决这个问题。120例常规处理的组织学未分化的大细胞人类肿瘤的组织切片与癌与淋巴瘤和/或黑色素瘤的组织学鉴别诊断进行了染色的单克隆抗角蛋白AE 1，单克隆抗白细胞共同抗原PD 7/26和2B 11，和兔抗S-100蛋白组成的抗体组。仅纳入常规形态学检查无法诊断的病例。PD 7/16和/或2B 11阳性61例（支持性淋巴瘤），AE 1阳性17例（支持性癌），抗S-100阳性25例（支持性黑色素瘤）。17例肿瘤与任何检测组抗体均无反应。没有肿瘤与针对两种或多种不同抗原的抗体反应。这些结果表明，该组抗体对组织学未分化肿瘤具有良好的特异性（100%）和灵敏度（86%）。这些结果在两个层面上是显著的：第一，作为评估间变性肿瘤抗体的小组方法的测试，第二，作为作者在此类病例中采用的特定小组的诊断效用的证明。
The evaluation of antibodies for diagnostic purposes on routinely processed sections of histologically undiagnosable neoplasms presents special problems. The authors have addressed this problem by constructing a panel of putatively mutually exclusive antibodies and testing it on sections of anaplastic neoplasms. Tissue sections of 120 routinely processed histologically undifferentiated large cell human neoplasms with a histologic differential diagnosis of carcinoma versus lymphoma and/or melanoma were stained with a panel of antibodies composed of monoclonal antikeratin AE1, monoclonal antileukocyte common antigens PD7/26 and 2B11, and rabbit anti-S-100 protein. Only cases not diagnosable by routine morphologic examination were included. PD7/16 and/or 2B11 were positive in 61 cases (supporting lymphoma), AE1 was positive in 17 cases (supporting carcinoma), and anti-S-100 was positive in 25 cases (supporting melanoma). Seventeen neoplasms failed to react with any of the panel antibodies. None of the neoplasms reacted with antibodies directed against two or more different antigens. These results indicate excellent specificity (100%) and good sensitivity (86%) of the panel antibodies on histologically undifferentiated neoplasms. These results are significant on two levels: first, as a test of the panel approach to evaluate antibodies on anaplastic neoplasms, and, second, as a demonstration of the diagnostic utility of the specific panel the authors have employed in such cases.