Intracellular calcium release channel expression during embryogenesis
Intracellular calcium release channel expression during embryogenesis
复制标题
DOI：
10.1006/dbio.1998.9120
复制
发表时间：
1999-02-15
影响因子：
2.7
通讯作者：
Marks, AR
中科院分区：
文献类型：
--
作者：
Rosemblit, N;Moschella, MC;Marks, AR
细胞内钙离子通过肌醇1，4，5-三磷酸受体(IP3R)或兰尼定受体(RyR)释放，在几乎所有类型的细胞中激活了广泛的信号通路。在本研究中，我们证明了在胚胎发育的早期，IP3R基因和功能性的IP3门控钙释放通道广泛表达于小鼠胚胎的几乎所有组织。随着器官发生的进行，更多的专化RyR通道在许多细胞类型中表达，细胞内钙释放的触发机制也变得更加多样化，包括IP3依赖、电压依赖和钙离子诱导的钙释放。随着发育的进行，几乎所有类型的细胞都继续表达IP3R通道，但在包括骨骼肌和心肌在内的兴奋性细胞中，主要的钙释放通道是RyR。这种从主要由IP3介导的细胞内钙释放途径转变为IP3介导和非IP3介导的钙释放途径，这与数据显示IP3R在细胞增殖和凋亡中发挥重要调节作用是一致的，而RyR是包括肌肉收缩在内的其他细胞功能所必需的。(C)1999年学术出版社。
The release of intracellular calcium (Ca2+) via either inositol 1,4,5-trisphosphate receptors (IP3R) or ryanodine receptors (RyR) activates a wide variety of signaling pathways in virtually every type of cell. In the present study we demonstrate that at early stages of development IP3R mRNA and functional IP3 gated Ca2+ release channels are widely expressed in virtually all tissues in murine embryos. As organogenesis proceeds, more specialized RyR channels are expressed in many cell types and the triggering mechanisms for intracellular Ca2+ release become more diverse to include IP3-dependent and voltage-dependent and Ca2+-induced Ca2+ release. As development proceeds virtually all cell types continue to express IP3R channels but in excitable cells including skeletal and cardiac muscles the major Ca2+ release channels are RyRs. This developmental switch from predominantly IP3-mediated to both IP3-mediated and IP3-independent pathways for intracellular Ca2+ release is consistent with data showing that IP3R plays an important regulatory role in cellular proliferation and apoptosis, whereas RyR is required for other cellular functions including muscle contraction. (C) 1999 Academic Press.