Genetically distinct astrocytic and oligodendroglial components in oligoastrocytomas
Genetically distinct astrocytic and oligodendroglial components in oligoastrocytomas
复制标题
DOI：
10.1007/s00401-006-0142-0
复制
发表时间：
2007-02-01
影响因子：
12.7
通讯作者：
Ren, Zhi-Ping
中科院分区：
文献类型：
--
作者：
Qu, Mingqi;Olofsson, Tommie;Ren, Zhi-Ping
少突星形细胞瘤是由肿瘤性星形细胞和少突胶质细胞的混合物组成的胶质肿瘤。少突星形细胞瘤的遗传改变包括染色体1 p和/或19 q的杂合性丢失（洛1 p/19 q），通常发生在少突胶质细胞瘤中，以及TP 53的突变，经常发生在星形细胞瘤中。为了研究这些肿瘤细胞类型在寡星形细胞瘤是否有不同的遗传谱，我们检查了两个不同的组成部分寡星形细胞瘤的比较，与组织学诊断的特定肿瘤区域的洛1 p/19 q和TP 53突变，通过使用显微切割技术。我们发现了1 p和19 q的各种丢失标记，以及肿瘤样本中存在两种不同的TP 53突变。在大多数情况下（9/11），少突胶质细胞和星形细胞成分的一个单独的少突星形细胞瘤显示相同的基因型。我们提出了两例双相寡星形细胞瘤异常的结果，这表明在这些肿瘤的遗传和形态不同的肿瘤细胞克隆共存。在一个病例中，肿瘤的少突胶质细胞部分显示LOH 19 q，而星形胶质细胞部分显示TP 53突变（密码子273）。在另一个病例中，我们在少突胶质细胞成分中发现了洛1 p/19 q，但在肿瘤的星形细胞成分中染色体1 p上有两个保留区域。没有证据表明，在同一个肿瘤的同一形态区域内，两种基因型变化的肿瘤细胞共存。我们的样本中的两例双相少突星形细胞瘤在肿瘤的星形细胞和少突胶质部分显示出不同的基因型，这表明少突星形细胞瘤的不同成分可能来源于肿瘤转化过程中的不同细胞克隆。
Oligoastrocytomas are glial tumours consisting of a mixture of neoplastic astrocytic and oligodendroglial cells. Genetic alterations of oligoastrocytomas include loss of heterozygosity of chromosomes 1p and/or 19q (LOH 1p/19q), typically occurring in oligodendrogliomas, and mutations of TP53, frequently occurring in astrocytomas. To investigate whether these neoplastic cell types in oligoastrocytomas have different genetic profiles, we examined the two different components of oligoastrocytomas in comparison with the histological diagnosis of the specific tumour area for LOH 1p/19q and TP53 mutations by using microdissection technique. We found a variety of lost markers for 1p and 19q, and the presence of two different TP53 mutations in the tumour samples. In the majority of cases (9/11), the oligodendroglial and astrocytic components of an individual oligoastrocytoma displayed the same genotype. We present two cases of biphasic oligoastrocytomas with aberrant findings, suggesting the coexistence of genetically and morphologically distinct tumour cell clones in these tumours. In one case, the oligodendroglial part of the tumour showed LOH19q, whereas the astrocytic part showed TP53 mutation (codon 273). In another case, we found LOH 1p/19q in the oligodendroglial component, but two retained areas on chromosome 1p in the astrocytic component of the tumour. No evidence was found for the coexistence of tumour cells with the two genotypical changes within the same morphological region of one individual tumour. The two cases of biphasic oligoastrocytomas in our sample that display a different genotype in the astrocytic and oligodendroglial part of the tumour show that different components of an oligoastrocytoma may be derived from different cell clones during neoplastic transformation.