Composition and diversity of the duodenal mucosa-associated microbiome in children with untreated coeliac disease
Composition and diversity of the duodenal mucosa-associated microbiome in children with untreated coeliac disease
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DOI：
10.3109/00365521.2013.775666
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发表时间：
2013-05-01
影响因子：
1.9
通讯作者：
Mearin, M. Luisa
中科院分区：
文献类型：
--
作者：
de Meij, Tim G. J.;Budding, Andries E.;Mearin, M. Luisa
背景资料。肠道微生物群可能在乳糜泻(CD)的发病机制中起一定作用。对比患有和不患有CD的儿童的肠道微生物群的研究是相互矛盾的。瞄准。比较CD初发组和非CD对照组十二指肠黏膜相关微生物组的组成和多样性，鉴定诊断时与CD相关的肠道细菌。方法：研究方法。应用16S-23S间隔区(IS)分析方法IS-PRO分析了42例患儿(21例未经治疗的CD和21例年龄匹配的对照)小肠活检组织的总微生物组图谱。结果。两组患者均表现出相似的粘膜相关微生物群格局和多样性，其中链球菌、乳杆菌和梭状芽孢杆菌属的浓度较高。结论。十二指肠黏膜相关微生物组的组成和多样性在未经治疗的CD儿童和对照组儿童之间没有差别。十二指肠粘膜相关细菌似乎在CD的发病机制中不起重要作用。
Background. Intestinal microbiome may play a role in the pathogenesis of coeliac disease (CD). Studies comparing intestinal microbiome in children with and without CD are contradictory. Aim. To compare the composition and diversity of the duodenal mucosa-associated microbiome in children with untreated CD and control children without CD and to identify specific gut bacteria associated with CD at diagnosis. Methods. Total microbiome profile in small bowel biopsies of 42 children (21 with untreated CD and 21 age-matched controls) were analyzed by means of IS-pro, a 16S-23S interspacer (IS) region-based profiling method. Results. Both groups showed a similar mucosa-associated microbiome pattern and diversity, with high concentrations of the genera Streptococcus, Lactobacillus, and Clostridium. Conclusion. Mucosa-associated duodenal microbiome composition and diversity did not differ between children with untreated CD and control children. Duodenal mucosa-associated bacteria do not seem to play an important role in the pathogenesis of CD.