Application of microalgae hydrolysate as a fermentation medium for microbial production of 2-pyrone 4,6-dicarboxylic acid
Application of microalgae hydrolysate as a fermentation medium for microbial production of 2-pyrone 4,6-dicarboxylic acid
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DOI：
10.1016/j.jbiosc.2017.12.026
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发表时间：
2018-06-01
影响因子：
2.8
通讯作者：
Takahashi, Kenji
中科院分区：
文献类型：
--
作者：
Htet, April N.;Noguchi, Mana;Takahashi, Kenji
以微藻的实际生物量作为微生物生产2-吡喃酮-4，6-二元酸(PDC)的发酵底物。将酸解的绿色微藻小球藻(水解液)稀释至葡萄糖浓度为2g/L，并添加Luria-Bertani(Luria-Bertani)(Luria-Bertani(Luria Bertani，Luria-Ber以藻水解液为发酵源，重组大肠杆菌生产PDC的产量为0.43g/L，得率为20.1%；而在添加葡萄糖的LB型培养基中，PDC产量为0.19g/L，得率为8.6%。为了评价藻类水解液单独作为大肠杆菌生长和PDC生产的发酵基质的潜力，从藻类水解物的培养基中降低了LB型液体培养基中的营养成分。有趣的是，即使在没有添加营养物质的情况下，PDC的产量也达到了0.17g/L，这与使用纯葡萄糖培养基加LB型营养物质的情况相当。在没有添加营养物质的情况下，利用高浓度的水解液，培养24 h，可获得1.22g/L的PDC，产率为16.1%。总体而言，艾氏假丝酵母具有作为微生物生产PDC的高性价比发酵底物的潜力。(C)2018年，日本生物技术学会。版权所有。
Actual biomass of microalgae was tested as a fermentation substrate for microbial production of 2-pyrone 4,6-dicarboxylic acid (PDC). Acid-hydrolyzed green microalgae Chlorella emersonii (algae hydrolysate) was diluted to adjust the glucose concentration to 2 g/L and supplemented with the nutrients of Luria-Bertani (LB) medium (tryptone 10 g/L and yeast extract 5 g/L). When the algae hydrolysate was used as a fermentation source for recombinant Escherichia coli producing PDC, 0.43 g/L PDC was produced with a yield of 20.1% (mol PDC/mol glucose), whereas 0.19 g/L PDC was produced with a yield of 8.6% when LB medium supplemented with glucose was used. To evaluate the potential of algae hydrolysate alone as a fermentation medium for E. coli growth and PDC production, the nutrients of LB medium were reduced from the algae hydrolysate medium. Interestingly, 0.17 g/L PDC was produced even without additional nutrient, which was comparable to the case using pure glucose medium with nutrients of LB medium. When using a high concentration of hydrolysate without additional nutrients, 1.22 g/L PDC was produced after a 24-h cultivation with the yield of 16.1%. Overall, C. emersonii has high potential as cost-effective fermentation substrate for the microbial production of PDC. (C) 2018, The Society for Biotechnology, Japan. All rights reserved.