Production of platelet-activating factor in glomeruli and cultured glomerular mesangial cells.
Production of platelet-activating factor in glomeruli and cultured glomerular mesangial cells.
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肾小球和培养的肾小球系膜细胞中血小板激活因子的产生。
DOI：
10.1152/ajprenal.1986.250.6.f1123
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Clay,KL
中科院分区：
文献类型：
--
作者：
Schlondorff,D;Goldwasser,P;Neuwirth,R;Satriano,JA;Clay,KL
血小板活化因子（PAF; 1-O-烷基-2-乙酰基-sn-甘油-3-磷酸胆碱）导致离体肾小球和培养的系膜细胞收缩，并伴随引起花生四烯酸的释放和前列腺素E2（PGE 2）的形成。肾脏和分离的肾小球也可以产生具有PAF生物活性的物质。因此，我们研究了大鼠离体肾小球和培养的肾小球系膜细胞形成PAF的能力。分离的肾小球和培养的系膜细胞都能将1-O-烷基-2-溶血-sn-甘油-3-磷酸胆碱（[3 H]lyso-PAF）转化为标记产物，并在薄层色谱（TLC）和高压液相色谱（HPLC）上与真正的PAF共迁移。用兔血小板聚集作为PAF的生物测定，我们发现分离的肾小球产生4 +/- 2 pmol/mg PAF样物质的肾小球蛋白，当用A23187（10（-5）M）刺激30分钟时，系膜细胞产生30 +/-8 pmol/mg细胞蛋白。系膜细胞产生的PAF物质的主要种类被鉴定为1-O-十六烷基-2-乙酰基-在HPLC分离之后，接着进行快速原子轰击和气相色谱-质谱法，得到sn-甘油-3-磷酸胆碱。这些结果表明，肾小球系膜细胞可以产生PAF，这可能有助于局部调节肾小球功能。
Platelet-activating factor (PAF; 1-O-alkyl-2-acetyl-sn-glycero-3-phosphocholine) results in contraction of isolated glomeruli and cultured mesangial cells and concomitantly causes release of arachidonic acid and prostaglandin E2 (PGE2) formation. The kidney and isolated glomeruli can also generate material that has PAF bioactivity. We therefore examined the capacity of isolated renal glomeruli and cultured glomerular mesangial cells from rats to form PAF. Both isolated glomeruli and cultured mesangial cells transformed 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine ([3H]lyso-PAF) into a labeled product comigrating both on thin-layer chromatography (TLC) and high-pressure liquid chromatography (HPLC) with authentic PAF. Using rabbit platelet aggregation as bioassay for PAF, we found that isolated glomeruli produced 4 +/- 2 pmol/mg glomerular protein of PAF-like material, and mesangial cells produced 30 +/- 8 pmol/mg cell protein when stimulated with A23187 (10(-5) M) for 30 min. The major species of the PAF material produced by mesangial cells was identified as 1-O-hexadecyl-2-acetyl-sn-glycero-3-phosphocholine after HPLC separation, followed by fast atom bombardment and gas chromatography-mass spectrometry. These results show that glomerular mesangial cells can produce PAF, which could contribute locally to the regulation of glomerular function.