Conjugative transfer by the virulence system of Legionella pneumophila
Conjugative transfer by the virulence system of Legionella pneumophila
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DOI：
10.1126/science.279.5352.873
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发表时间：
1998-02-06
期刊：
影响因子：
56.9
通讯作者：
Isberg, RR
中科院分区：
文献类型：
--
作者：
Vogel, JP;Andrews, HL;Isberg, RR
嗜肺军团菌是军团肺炎的病原体，通过阻止吞噬体-溶酶体融合在肺泡巨噬细胞内复制。在这里，分离出了大量称为 dof（细胞器运输缺陷）的突变体，这些突变体由于细菌无法改变巨噬细胞的内吞途径而无法在细胞内复制。点毒力基因编码了一个大的假定膜复合物，该复合物充当分泌系统，能够将质粒DNA从一个细胞转移到另一个细胞。
Legionella pneumophila, the causative agent of Legionnaires' pneumonia, replicates within alveolar macrophages by preventing phagosome-lysosome fusion. Here, a large number of mutants called dof (defective for organelle trafficking) that were unable to replicate intracellularly because of an inability of the bacteria to alter the endocytic pathway of macrophages were isolated. The dot virulence genes encoded a large putative membrane complex that functioned as a secretion system that was able to transfer plasmid DNA from one cell to another.