Controlled Surface Silanization for Actin-Myosin and Biocompatibility of New Polymer Resists
Controlled Surface Silanization for Actin-Myosin and Biocompatibility of New Polymer Resists
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DOI：
10.1021/acs.langmuir.8b01415
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发表时间：
2018-07-31
期刊：
影响因子：
3.9
通讯作者：
Mansson, Alf
中科院分区：
文献类型：
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作者：
Lindberg, Frida W.;Norrby, Marlene;Mansson, Alf
基于分子马达的纳米器件需要有组织的细胞骨架丝引导沿着运动促进轨道，由运动抑制壁限制。一种提高轨道上的运动性质量的方法，特别是在细丝速度方面，而且还有运动细丝的分数，是优化表面疏水性。我们已经研究了可能实现这一点的肌动蛋白肌球蛋白II电机系统上的三甲基氯硅烷（TMCS）衍生的二氧化硅表面被用作通道地板的纳米器件。我们还研究了两种新的聚合物抗蚀剂，TU 7（用于纳米压印光刻）和CSAR 62（用于电子束和深紫外光刻），被用作通道壁抑制运动的能力。我们开发了一种化学气相沉积工具，用于在受控环境中对SiO2表面进行硅烷化，以实现不同的表面疏水性（通过水接触角测量）。与以前的工作相比，我们能够通过仅使用一种类型的硅烷改变硅烷化时间和腔室压力来制造宽范围的接触角。这导致了硅烷化过程的显著改善，在表面上产生可预测的接触角，从而在速度方面产生可预测质量的重肌球蛋白（HMM）驱动的肌动蛋白运动。我们观察到在10-86度的范围内的长丝滑动速度和接触角之间的高度相关性，扩大了先前研究的范围。我们发现，TU 7表面上的滑动速度是上级的CSAR 62表面，尽管类似的接触角。此外，在硅烷化之前，我们能够通过等离子体氧处理来抑制TU 7和CSAR 62上的运动性。这些结果进行了讨论，在以前提出的HMM的表面吸附机制和它们的关系，水接触角。此外，结果被认为是肌动蛋白-肌球蛋白的功能方面具有上级性能的肌动蛋白-肌球蛋白的纳米器件的发展。
Molecular motor-based nanodevices require organized cytoskeletal filament guiding along motility-promoting tracks, confined by motility-inhibiting walls. One way to enhance motility quality on the tracks, particularly in terms of filament velocity but also the fraction of motile filaments, is to optimize the surface hydrophobicity. We have investigated the potential to achieve this for the actin myosin II motor system on trimethylchlorosilane (TMCS)-derivatized SiO2 surfaces to be used as channel floors in nanodevices. We have also investigated the ability to supress motility on two new polymer resists, TU7 (for nanoimprint lithography) and CSAR 62 (for electron beam and deep UV lithography), to be used as channel walls. We developed a chemical-vapor deposition tool for silanizing SiO2 surfaces in a controlled environment to achieve different surface hydrophobicities (measured by water contact angle). In contrast to previous work, we were able to fabricate a wide range of contact angles by varying the silanization time and chamber pressure using only one type of silane. This resulted in a significant improvement of the silanization procedure, producing a predictable contact angle on the surface and thereby predictable quality of the heavy meromyosin (HMM)-driven actin motility with regard to velocity. We observed a high degree of correlation between the filament sliding velocity and contact angle in the range 10-86 degrees, expanding the previously studied range. We found that the sliding velocity on TU7 surfaces was superior to that on CSAR 62 surfaces despite similar contact angles. In addition, we were able to suppress the motility on both TU7 and CSAR 62 by plasma oxygen treatment before silanization. These results are discussed in relation to previously proposed surface adsorption mechanisms of HMM and their relationship to the water contact angle. Additionally, the results are considered for the development of actin-myosin based nanodevices with superior performance with respect to actin-myosin functionality.