Blocking estradiol synthesis affects memory for songs in auditory forebrain of male zebra finches.
Blocking estradiol synthesis affects memory for songs in auditory forebrain of male zebra finches.
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DOI：
10.1097/wnr.0b013e3283588b61
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发表时间：
2012-11-14
期刊：
影响因子：
1.7
通讯作者：
Vicario DS
中科院分区：
文献类型：
--
作者：
Yoder KM;Lu K;Vicario DS
最近研究表明，雌二醇（E2）可以调节鸣禽前脑听觉区（尾内侧巢皮层（NCM））的感觉加工。当一只鸟听到同种歌曲时，NCM中的E2局部增加，其中神经元表达芳香酶，该酶从前体和雌激素受体合成E2。NCM中的听觉反应表现出一种形式的神经元记忆：重复播放同种个体独特的学习发声，诱导对每个发声的神经反应的持久刺激特异性适应（SSA）。为了测试E2在听觉记忆中的作用，我们用芳香酶抑制剂法倔唑（FAD）或生理盐水处理成年雄性斑胸草雀（n=16）8天。然后，我们将他们暴露于“训练”歌曲，6小时后，用NCM中的16个微电极阵列记录多单位听觉反应。适应率（SSA的措施）的训练和新的歌曲播放计算，使用既定的方法，提供一个衡量神经元的记忆。从FAD处理的鸟类的录音显示显着降低的记忆的训练歌曲相比，盐水处理的控制，而听觉处理的新的歌曲没有不同的治疗组。此外，FAD并没有改变反应偏向于同种异种歌曲刺激。我们的研究结果表明，E2耗竭影响鸣禽NCM的发声神经元记忆，并建议E2在听觉加工和记忆通信信号中起着必要的作用。
Estradiol (E2) has recently been shown to modulate sensory processing in an auditory area of the songbird forebrain, the caudomedial nidopallium (NCM). When a bird hears conspecific song, E2 increases locally in NCM, where neurons express both the aromatase enzyme that synthesizes E2 from precursors and estrogen receptors. Auditory responses in NCM show a form of neuronal memory: repeated playback of the unique learned vocalizations of conspecific individuals induces long-lasting stimulus specific adaptation (SSA) of neural responses to each vocalization. In order to test the role of E2 in this auditory memory, we treated adult male zebra finches (n=16) with either the aromatase inhibitor Fadrozole (FAD) or saline for 8 days. We then exposed them to “training” songs and, 6h later, recorded multiunit auditory responses with an array of 16 microelectrodes in NCM. Adaptation rates (a measure of SSA) to playbacks of training and novel songs were computed, using established methods, to provide a measure of neuronal memory. Recordings from FAD-treated birds showed significantly reduced memory for the training songs compared to saline-treated controls, while auditory processing for novel songs did not differ between treatment groups. In addition, FAD did not change the response bias in favor of conspecific over heterospecific song stimuli. Our results show that E2 depletion affects the neuronal memory for vocalizations in songbird NCM, and suggest that E2 plays a necessary role in auditory processing and memory for communication signals.