Phenotypic stability of murine tumor cells in vitro and in vivo.
Phenotypic stability of murine tumor cells in vitro and in vivo.
复制标题
小鼠肿瘤细胞的体外和体内表型稳定性。
DOI：
10.1093/jnci/68.6.957
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Edmund J. Lovett
中科院分区：
文献类型：
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作者：
J. Varani;Edmund J. Lovett
从异质亲本肿瘤（在雌性C57 BL/6近交系小鼠中诱导）中分离在同基因小鼠中可移植性不同的鼠纤维肉瘤细胞，并通过连续传代维持培养。区分高和低可移植品系的几种生物学特性（包括蠕动、运动性和胰凝乳蛋白酶样酯酶活性水平），以及不区分这些品系的特性（例如，体外生长速率以及蛋白酶和糖苷酶活性水平），在2年内定期测量。以相同的时间间隔评价诱导原发性肿瘤和诱导这些肿瘤转移的能力。高和低可移植系也以致瘤剂量移植到同基因小鼠中。在培养物中重新建立来自动物中诱导的原发性和转移性肿瘤的分离株，并检查第5、10、20和30代的各种特征。在整个2年的观察期内，连续培养的细胞的性质保持稳定。肿瘤分离株在培养物中重建后立即显示出一些调节的证据，但到第10代时，它们似乎与原型亲本系相同。这些数据表明，纤维肉瘤细胞不经历连续的表型“漂移”，如已经提出的在动物或培养物中通过连续传代维持的其他肿瘤系中发生的。
Murine fibrosarcoma cells that vary in their transplantability in syngeneic mice were isolated from a heterogeneous parent tumor (induced in a female C57BL/6 inbred mouse) and maintained in culture by serial passage. Several biologic properties that discriminate between the high- and low-transplantable lines (including adhesiveness, motility, and levels of chymotrypsin-like esterase activity), as well as properties that do not separate these lines (e.g., in vitro growth rates and levels of protease and glycosidase activities), were measured at periodic intervals over 2 years. Ability to induce primary tumors and to induce metastases from these tumors were evaluated at the same intervals. The high- and low-transplantable lines were also transplanted into syngeneic mice at tumorigenic doses. Isolates from primary and metastatic tumors induced in the animals were reestablished in culture and examined for the various characteristics of passages 5, 10, 20, and 30. The properties of the cells maintained continually in culture remained stable throughout the 2-year observation period. Tumor isolates showed some evidence of modulation immediately after reestablishment in culture, but by passage 10 they appeared to be identical to the prototype parent lines. These data show that the fibrosarcoma cells do not undergo continual phenotypic "drift" as has been suggested to occur with other tumor lines maintained by serial passage in animals or in culture.