Distribution of interspersed repeats (Alu and Kpn) on NotI restriction fragments of human chromosome 21.
Distribution of interspersed repeats (Alu and Kpn) on NotI restriction fragments of human chromosome 21.
复制标题
人类 21 号染色体 NotI 限制性片段上散布重复序列（Alu 和 Kpn）的分布。
DOI：
10.1073/pnas.89.3.1080
复制
发表时间：
1992
影响因子：
11.1
通讯作者：
Smith,CL
中科院分区：
文献类型：
--
作者：
Sainz,J;Pevny,L;Wu,Y;Cantor,CR;Smith,CL
以交叉重复序列（Interspersed repeats sequences， Alu和Kpn）为探针，检测了来自杂交细胞系WAV17的人类21号染色体Not I限制性内切片段。共鉴定了40个不同的Not I片段，大小从小于0.05 Mb到7.0 Mb不等。这些片段的总长度为47.3 Mb。这个长度提供了染色体的最小大小和创建一个完整的限制图谱所需的最小片段数量的估计。Not I片段的平均长度为1.2 Mb。Alu和Kpn片段并不总是一致的：Kpn中检测到2.9 Mb的片段，而Alu中没有检测到，Alu中检测到13个片段，范围从0.05 Mb到5.6 Mb不等，而Kpn中没有检测到；剩余的26个片段，占总长度的75% (35.3 Mb)，用两个重复探针检测。如此多的不一致片段的存在和片段杂交信号强度的高变化表明Kpn和Alu重复序列的分布非常不均匀。
Interspersed repeated sequences (Alu and Kpn) were used as probes to detect a set of Not I restriction fragments of human chromosome 21 from the hybrid cell line WAV17. Forty different Not I fragments, ranging in size from less than 0.05 megabase (Mb) to 7.0 Mb, were identified. The total length of these fragments was 47.3 Mb. This length provides an estimate of the minimum size of the chromosome and a minimum number of fragments to be ordered to create a complete restriction map. The average length Not I fragment is 1.2 Mb. Alu and Kpn fragments are not always coincident: a 2.9-Mb fragment is detected with Kpn but not with Alu, and 13 fragments, ranging from less than 0.05 Mb to 5.6 Mb, are detected with Alu but not with Kpn; the 26 remaining fragments, covering 75% (35.3 Mb) of the total length, are detected with both repetitive probes. The presence of so many noncoincident fragments and the high variation of the hybridization signal intensities of the fragments suggest a very nonuniform distribution of Kpn and Alu repeats.