Polymorphism of somatolactin-producing cells in the goldfish pituitary: immunohistochemical investigation for somatolactin-α and -β
Polymorphism of somatolactin-producing cells in the goldfish pituitary: immunohistochemical investigation for somatolactin-α and -β
复制标题
金鱼垂体中生长乳素产生细胞的多态性：生长乳素-α和-β的免疫组织化学研究
DOI：
10.1007/s00441-012-1435-3
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发表时间：
2012
影响因子：
3.6
通讯作者：
Azuma M
中科院分区：
文献类型：
--
作者：
Imai S;Kano M;Nonoyama K;Ebihara S;Azuma M
生长激素（SL）是一种垂体激素，属于腺垂体激素的生长激素/催乳素家族。在硬骨鱼中，SL 由一或两个旁系同源基因编码，即 SL-α 和 -β。我们之前的研究表明，垂体腺苷酸环化酶激活多肽会刺激培养金鱼垂体细胞释放 SL，而黑色素浓缩激素则抑制这种释放。与其他鱼类一样，金鱼也具有 SL-α 和 -β。然而，到目前为止，还没有任何有用的方法可以通过免疫学方法检测该物种中各自的 SL。为了实现这一目标，我们针对从金鱼 SL-α 和 -β cDNA 序列推导的合成肽片段制备了兔抗血清。使用这些抗血清，我们观察到金鱼垂体中的腺垂体细胞显示出 SL-α 和 β 样免疫反应性，尤其是中间部 (PI)。 PI 中的几个细胞表现出 SL-α 和 SL-β 样免疫反应性的共定位。然后，使用单细胞聚合酶链反应和激光显微切割，我们检查了腺垂体细胞中的 SL-α 和 -β 基因表达，显示出 SL-α- 或 -β 样免疫反应性。在培养的垂体细胞中，我们观察到了三种类型的细胞：那些拥有 SL-α、-β 或两者的转录本的细胞。这些结果表明金鱼垂体中产生 SL 的细胞存在多态性。
Somatolactin (SL) is a pituitary hormone belonging to the growth hormone/prolactin family of adenohypophyseal hormones. In teleost fish, SL is encoded by one or two paralogous genes, namely SL-α and -β. Our previous studies have revealed that pituitary adenylate-cyclase-activating polypeptide stimulates SL release from cultured goldfish pituitary cells, whereas melanin-concentrating hormone suppresses this release. As in other fish, the goldfish possesses SL-α and -β. So far, however, no useful means of detecting the respective SLs immunologically in this species has been possible. In order to achieve this aim, we raised rabbit antisera against synthetic peptide fragments deduced from the goldfish SL-α and -β cDNA sequences. Using these antisera, we observed adenohypophyseal cells showing SL-α- and -β-like immunoreactivities in the goldfish pituitary, especially the pars intermedia (PI). Several cells in the PI showed the colocalization of SL-α- and -β-like immunoreactivities. Then, using single-cell polymerase chain reaction with laser microdissection, we examined SL-α and -β gene expression in adenohypophyseal cells showing SL-α- or -β-like immunoreactivity. Among cultured pituitary cells, we observed three types of cell: those that possess transcripts of SL-α, -β, or both. These results suggest a polymorphism of SL-producing cells in the goldfish pituitary.