Effect of Nanofeatured Topography on Ceria-Stabilized Zirconia/Alumina Nanocomposite on Osteogenesis and Osseointegration
Effect of Nanofeatured Topography on Ceria-Stabilized Zirconia/Alumina Nanocomposite on Osteogenesis and Osseointegration
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DOI：
10.11607/jomi.4366
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发表时间：
2017-01-01
影响因子：
2
通讯作者：
Baba, Kazuyoshi
中科院分区：
文献类型：
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作者：
Oshima, Yoko;Iwasa, Fuminori;Baba, Kazuyoshi
目的：研究氢氟酸(HF)处理后Ce-TZP/Al(2)O(3)纳米结构氧化锆/氧化铝复合材料的成骨和骨整合能力。材料和方法：在酸蚀钛片和Ce-TZP/Al(2)O(3)片上培养MC3T3-E1成骨细胞，比较4%HF(0%)、4%HF(4%)和55%HF(55%)处理24小时后的生物学反应。在大鼠股骨内植入AETi和Zr(55%)的微型种植体。在愈合2周和4周后，通过生物力学推入试验评价骨整合。结果：氧化锆(55%)表面呈现纳米形貌，具有更大的比表面积和粗糙度，并有广泛的地理凹陷，氧化铈-氧化锆晶体从表层消失，与生物矿化基质的表面形态相似。培养研究表明，成骨细胞的附着、增殖、铺展和功能表型，如碱性磷酸酶活性和骨相关基因的表达，在55%的锆表面上显著增加。在一个大鼠模型中，用生物力学推入试验测得的骨结合强度，锆种植体(55%)比AETi植入体强1.6倍。结论：纳米Ce-TZP/Al_2O_3表面可显著提高体外成骨反应和体内骨结合能力，有望作为一种新型种植材料应用于临床。
Purpose: The objective of this study was to evaluate the osteogenic and osseointegration capability of the Ce-tetragonal zirconia polycrystal (TZP)-based nanostructured zirconia/alumina composite (Ce-TZP/AI(2)O(3)) that was treated with hydrofluoric acid (HF). Materials and Methods: Osteogenic MC3T3-E1 cells were cultured on acid-etched titanium (AETi) disks and Ce-TZP/AI(2)O(3) disks without HF treatment (Zr[0%]), with 4% HF treatment (Zr[4%]), or with 55% HF treatment (Zr[55%]) for 24 hours, and biologic responses were compared among four conditions in vitro. Miniature implants of AETi and Zr(55%) were surgically placed in the femora of rats. Osseointegration was evaluated by a biomechanical push-in test after 2 and 4 weeks of healing. Results: The surface of Zr(55%) rendered nanofeatured topography with a greater surface area and roughness, and extensive geographical undercut as ceria-zirconia crystal disappeared from the superficial layer and was similar to the surface morphology of biomineralized matrices. Culture studies showed that the attachment, proliferation, spread, and functional phenotypes of osteogenic cells, such as alkaline phosphatase activity and bone-related gene expression, were remarkably increased on the Zr(55%) surface. The strength of osseointegration measured using the biomechanical push-in test in a rat model was stronger for Zr(55%) implants than for AETi implants by 1.6 fold. Conclusion: The nanostructured Ce-TZP/Al2O3 surface substantially enhanced the osteogenic response in vitro and the osseointegration capability in vivo, which suggest its potential clinical application as a novel implant material.