Dissolved organic carbon contamination induced by filters and storage bottles
Dissolved organic carbon contamination induced by filters and storage bottles
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DOI：
10.1016/s0043-1354(98)00407-2
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发表时间：
1999-06
期刊：
影响因子：
12.8
通讯作者：
S. C. Yoro;C. Panagiotopoulos;R. Sempéré
中科院分区：
文献类型：
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作者：
S. C. Yoro;C. Panagiotopoulos;R. Sempéré
为了优化实验室条件下海洋溶解有机碳（DOC）研究的实验方案，我们研究了mil - q水和0.2μm过滤海水在10d内不同质量过滤器和不同类型瓶子的潜在DOC释放量。结果表明：采用聚碳酸酯（孔径为0.2 μm和1μm）、聚四氟乙烯（孔径为0.5μm）和氧化铝（孔径为0.2 μm和0.02μm）等47-mm圆形过滤器，过滤后的前100 ml溶液中DOC污染显著，范围为12 ~ 42μM C；然而，2 - 4l milliq水的过滤使过滤器的DOC空白降低到2 - 5μ C。正如预期的那样，用0.5-3l milliq水冲洗预燃烧玻璃纤维过滤器（450°C, 6h）产生非常低的空白（1 - 4μ C）。我们发现，将ml - q水和0.2μm过滤的海水（250ml）放入1l-聚乙烯、聚碳酸酯和全氟烷氧基（PFA）聚四氟乙烯瓶中（15℃，暗处）10天，先用1%的盐酸清洗，然后用大量ml - q水大量冲洗，再放入预燃烧的玻璃瓶（450℃，6h）中，溶液（0 - 5μ C）的DOC污染非常低。尽管在船上条件下很难达到完全的无DOC污染实验，但这些结果强烈表明，为了达到与海洋环境中DOC研究相一致的空白水平，对材料进行适当的清洁程序是必不可少的。
To optimize experimental protocol for marine dissolved organic carbon (DOC) studies in laboratory conditions, we examined the potential DOC releases from filters of different qualities as well as those from different types of bottles during 10day-storage of Milli-Q water and 0.2μm-filtered seawater. The results showed that utilization of 47-mm circle filters including polycarbonate (0.2 and 1μm-pore size), polytetrafluoroethylene (PTFE: 0.5μm-pore size) and aluminum oxide (0.2 and 0.02μm-pore size) may induce significant DOC contamination ranging from 12 to 42μM C for the first hundred milliliters of the filtrated solutions. However, filtrations of 2 to 4l of Milli-Q water decreased the DOC blank of the filters to 2–5μM C. As expected, precombusted (450°C, 6h) glass fiber filters rinsed with 0.5–3l of Milli-Q water produced very low blanks (1–4μM C). We found that 10day-storage (15°C, in the dark) of Milli-Q water and 0.2μm-filtered seawater (250ml) into 1l-polyethylene, polycarbonate and perfluoalkoxy (PFA)-Teflon bottles, initially cleaned with HCl 1% and abondantly rinsed with copious amounts of Milli-Q water as well as in precombusted glass bottles (450°C, 6h) induced very low DOC contamination of the solutions (0–5μM C). Although complete DOC-free contamination experiment is difficult to reach particularly in shipboard conditions, these results strongly suggest that appropriate cleaning procedure of the material is essential to reach blank level compatible with DOC studies in marine environments.