A mouse model for poliovirus neurovirulence identifies mutations that attenuate the virus for humans.
A mouse model for poliovirus neurovirulence identifies mutations that attenuate the virus for humans.
复制标题
脊髓灰质炎病毒神经毒力的小鼠模型鉴定出能够减弱人类病毒的突变。
DOI：
10.1128/jvi.61.9.2917-2920.1987
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发表时间：
1987
影响因子：
5.4
通讯作者：
Racaniello,VR
中科院分区：
文献类型：
--
作者：
LaMonica,N;Almond,JW;Racaniello,VR
已知可降低人类神经毒力的 3 型脊髓灰质炎病毒基因组突变，当掺入小鼠适应的人类脊髓灰质炎病毒重组体时，同样会降低小鼠的神经毒力。在其基因组 5'-非编码区的核苷酸位置 472 处具有尿嘧啶的病毒重组体无法在小鼠大脑中复制。该位置带有胞嘧啶的病毒重组体对小鼠具有神经毒性。因此，小鼠脊髓灰质炎病毒的神经毒力可能被证明是定点诱变产生的新减毒突变遗传稳定性的有用指标。
A mutation in the genome of poliovirus type 3 that is known to reduce neurovirulence in humans similarly reduces neurovirulence in mice when incorporated into a mouse-adapted-human poliovirus recombinant. Viral recombinants with a uracil at nucleotide position 472 in the 5'-noncoding regions of their genomes are unable to replicate in the mouse brain. Viral recombinants with a cytosine at this position are neurovirulent in mice. Neurovirulence of poliovirus in mice may therefore prove to be a useful indicator of the genetic stability of new attenuating mutations created by site-directed mutagenesis.