ANTI-PHAGE ANTIBODIES INDUCED DURING PHAGE THERAPY MIGHT DECREASE EFFICACIES OF THERAPEUTIC PHAGES
ANTI-PHAGE ANTIBODIES INDUCED DURING PHAGE THERAPY MIGHT DECREASE EFFICACIES OF THERAPEUTIC PHAGES
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噬菌体治疗过程中诱导的抗噬菌体抗体可能会降低噬菌体治疗的功效
DOI：
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Sebastian Leptihn
中科院分区：
文献类型：
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作者：
Zelin Cui;Sebastian Leptihn
最近发表在2020年3月《自然微生物学》杂志上的一篇文章《严重金黄色葡萄球菌感染中噬菌体疗法的安全性》在I期临床试验中评估了噬菌体疗法的安全性。监测静脉给药后血中噬菌体的药理参数。在最初5天的给药开始时，在血样中观察到完整的噬菌体，通过菌斑分析进行评估。然而，在接下来的9天里，没有发现完整的噬菌体，而它们的DNA却可以用实时荧光聚合酶链式反应检测到，这提出了一个问题，为什么在后来的给药过程中没有检测到完整的噬菌体颗粒。在这里，我们假设在最初几轮噬菌体疗法中诱导的抗体可能导致治疗性病毒灭活。
A recent article “Safety of bacteriophage therapy in severe Staphylococcus aureus infection” published in March .2020 in Nature Microbiology evaluated the safety of phage therapy in phase I clinical trials. The pharmaceutical .parameters of the phages in the blood which administered by intravenous injection were monitored. At the beginning of the administration during the initial 5 days, intact phages were observed in blood samples, assessed by .bacterial plaque assays. However, in the subsequent 9 days no intact phages were found, while their DNA could .be detected by using real-time PCR, posing the question why no intact phage particles are being detected in the .later course of administrations. Here, we postulate that antibodies induced during the initial rounds of phage therapy might lead to inactivation of the therapeutic viruses.