Enhanced production of L(+)-lactic acid from corn starch in a culture of Rhizopus oryzae using an air-lift bioreactor
Enhanced production of L(+)-lactic acid from corn starch in a culture of Rhizopus oryzae using an air-lift bioreactor
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DOI：
10.1016/s0922-338x(97)82063-6
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发表时间：
1997-01-01
期刊：
影响因子：
--
通讯作者：
Okabe, M
中科院分区：
文献类型：
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作者：
Yin, PM;Nishina, N;Okabe, M
用8株根霉菌株和不同的培养条件对L(+)-乳酸的产生进行了研究。米根霉NRRL395的L(+)-乳酸产量最高。最佳生产条件为：玉米淀粉120g/L，硫酸铵1.35g/L，添加少量的无机盐(磷酸钾、硫酸镁、硫酸锌)。在此优化条件下，用L 3型气升式生物反应器成功地再现了摇瓶培养的数据。在3号气升式生物反应器中，L(+)-乳酸的产率(以初始碳源浓度计)为85%，最终浓度为102g/L。采用过滤、活性碳处理、超滤、真空蒸发浓缩4个步骤回收乳酸，收率(以发酵液中乳酸含量计)为90%。经高效液相色谱鉴定，纯化的乳酸为100%L(+)型。
L(+)-Lactic acid production was investigated using 8 Rhizopus strains and various culture conditions. Rhizopus oryzae NRRL395 showed the highest yield of L(+)-lactic acid among various Rhizopus strains. The optimum production medium contained the following: corn starch, 120 g/l; ammonium sulfate, 1.35 g/l; small amounts of mineral salts (potassium phosphate, magnesium sulfate and zinc sulfate). The data obtained for the flask culture were successfully reproduced using a 3 l air-lift bioreactor under the optimum conditions. The yield (based on initial carbon source concentration) and final concentration of L(+)-lactic acid were 85% and 102 g/l, respectively, in the 3 l air-lift bioreactor. The lactic acid was recovered with a yield (based on lactic acid containing in broth) of 90% using 4 steps, i.e., filtration, active carbon treatment, ultra-filtration and concentration by vacuum evaporation. The lactic acid purified was 100% L(+)-form as judged by HPLC.